Diet enriched with procyanidins enhances antioxidant activity and reduces myocardial post-ischaemic damage in rats
Diet enriched with procyanidins enhances antioxidant activity and reduces myocardial post-ischaemic damage in rats
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DOI：
10.1016/s0024-3205(98)00605-5
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发表时间：
1999-01-15
期刊：
影响因子：
6.1
通讯作者：
Morazzoni, P
中科院分区：
文献类型：
--
作者：
Facino, RM;Carini, M;Morazzoni, P
本研究旨在研究葡萄籽原花青素（一种多酚类抗氧化剂的标准混合物）对大鼠缺血/再灌注昏迷后3周心脏力学的影响。幼龄和老年雄性大鼠分别饲喂原花青素（1:3 w/w）与大豆卵磷脂（2.4%）复合的饲粮；对照动物（幼年和老年）给予等量的卵磷脂，另外两组动物给予标准饮食。在治疗结束时，测定中度缺血（血流减少至1 ml/min，持续20 min）和再灌注（15 ml/min，持续30 min）各组动物血浆和心脏中的总血浆抗氧化防御（TRAP）、维生素E、抗坏血酸和尿酸。年轻大鼠和老年大鼠补充原花青素后，再灌注结束时左心室发育压（LVDP）恢复为缺血前的93% （p < 0.01）和74% (p < 0.01)，冠状动脉灌注压（CPP）维持在缺血前的水平。此外，肌酸激酶（CK）流出量被抑制在基线水平，而在缺血和再灌注期间，青年和老年大鼠心脏灌注液中的前列环素（6-酮- pgf (1 α)）增加了2倍。同时，原花青素显著提高血浆总抗氧化能力（年轻大鼠提高40%，老年大鼠提高30%）和血浆抗坏血酸水平，同时趋于降低维生素E水平；尿酸水平无显著差异。本研究结果表明，在大鼠（年轻和年老）中补充原花青素使心脏不容易受到缺血/再灌注损伤，这与血浆抗氧化活性的增加正相关。
Aim of this work was to study the efficacy of procyanidins from Vitis vinifera seeds, a standardized mixture of polyphenol antioxidants, on cardiac mechanics following ischemia/reperfusion stunning in the rat, after 3 weeks supplementation. Young and aged male rats were fed a diet enriched with procyanidins complexed (1:3 w/w) with soybean lecithin (2.4%); control animals (CTR-young and CTR-aged) received an equal amount of lecithin and 2 additional groups of animals the standard diet. At the end of the treatment, the total plasma antioxidant defense (TRAP), vitamin E, ascorbic acid and uric acid were determined in plasma and the hearts from all groups of animals subjected to moderate ischemia (flow reduction to 1 ml/min for 20 min) and reperfusion (15 ml/min for 30 min). In both young and aged rats supplemented with procyanidins the recovery of left ventricular developed pressure (LVDP) at the end of reperfusion was 93% (p < 0.01) and 74% (p < 0.01) of the preischemic values and the values of coronary perfusion pressure (CPP) were maintained close to those of the preischemic period. Also creatine kinase (CK) outflow was restrained to baseline levels, while a 2-fold increase in prostacyclin (6-keto-PGF(1 alpha)) in the perfusate from hearts of young and aged rats was elicited during both ischemia and reperfusion. In parallel, procyanidins significantly increased the total antioxidant plasma capacity (by 40% in young and by 30% in aged rats) and the plasma levels of ascorbic acid, while tend to reduce vitamin E levels; no significant differences were observed in uric acid levels. The results of this study demonstrate that procyanidins supplementation in the rat (young and aged) makes the heart less susceptible to ischemia/reperfusion damage and that this is positively associated to an increase in plasma antioxidant activity.