Immunohistochemical localization of senescence marker protein-30 (SMP30) in the submandibular gland and ultrastructural changes of the granular duct cells in SMP30 knockout mice
Immunohistochemical localization of senescence marker protein-30 (SMP30) in the submandibular gland and ultrastructural changes of the granular duct cells in SMP30 knockout mice
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DOI：
10.14670/hh-20.761
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发表时间：
2005-07-01
影响因子：
2
通讯作者：
Akita, M
中科院分区：
文献类型：
--
作者：
Ishii, K;Tsubaki, T;Akita, M
衰老标记蛋白-30（SMP 30）是一种钙调节蛋白，其随着衰老发生以雄激素非依赖性方式减少。酶标抗体技术已证明，SMP 30定位于导管（颗粒，闰管，和条纹管）的小鼠下颌下腺。免疫电镜显示，颗粒管细胞呈SMP 30强阳性，而柱细胞呈阴性。在SMP 30敲除（KO）小鼠中，颗粒管直径较小。在颗粒管细胞中观察到线粒体肿胀;然而，在柱细胞中没有观察到这种现象。α-异丙肾上腺素（一种β-肾上腺素能兴奋剂）给药后，在SMP 30-野生型（WT）小鼠的颗粒管细胞和粗面内质网扩张中存在大量小分泌颗粒;相比之下，在SMP 30-KO小鼠中观察到的变化很小。这些结果表明，SMP 30可能与颌下腺颗粒管细胞的信号转导途径密切相关。
Senescence Marker Protein-30 (SMP30) is a calcium-regulating protein that decreases in an androgen-independent manner as aging occurs. An enzyme-labeled antibody technique has demonstrated that SMP30 localized to the ducts (granular, intercalated, and striated ducts) of mouse submandibular glands. Immunoelectronmicroscopy demonstrated that the granular duct cells were strongly positive for SMP30, but that pillar cells in the granular duct were negative for the protein. In SMP30-knockout (KO) mice, the granular ducts were smaller in diameter. Swelling of mitochondria in the granular duct cells was observed; however, this phenomenon was not observed in the pillar cells. After administration of alpha-isoproterenol, a beta-adrenergic stimulant, a large numbers of small secretory granules were present in the granular duct cells and an expansion of the rough endoplasmic reticulum in SMP30-wild type (WT) mice; in contrast, little change was observed in SMP30-KO mice. These results suggest that SMP30 may be closely related to a signal transduction pathway in the granular duct cells of submandibular glands.