Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle.
Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle.
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DOI：
10.7554/elife.30963
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发表时间：
2018-01-29
期刊：
影响因子：
7.7
通讯作者：
Amack JD
中科院分区：
文献类型：
--
作者：
Dasgupta A;Merkel M;Clark MJ;Jacob AE;Dawson JE;Manning ML;Amack JD
如何协调调节上皮细胞的行为以塑造组织结构是生物学中的一个基本问题。库普弗氏囊泡(KV)是一种具有充液腔的暂时性器官，它提供了一个简单的系统来研究上皮形态发生过程中内在细胞机制和外力之间的相互作用。使用单个细胞的三维(3D)分析，我们识别出沿KV前后轴的不对称细胞体积变化，这与不对称细胞形状变化相一致。阻止离子通量可以阻止这些细胞体积的改变和细胞形状的改变。顶点模拟表明，细胞形状的变化不依赖于管腔的扩张。与这一预测一致，当KV腔生长因细胞粘连泄漏而失败时，KV细胞体积和形状的不对称变化正常发生。这些结果表明，离子通量介导了细胞体积的变化，从而导致了KV中不对称的细胞形状变化，并且这些上皮细胞形态的变化与管腔产生的力是分开的。
How epithelial cell behaviors are coordinately regulated to sculpt tissue architecture is a fundamental question in biology. Kupffer’s vesicle (KV), a transient organ with a fluid-filled lumen, provides a simple system to investigate the interplay between intrinsic cellular mechanisms and external forces during epithelial morphogenesis. Using 3-dimensional (3D) analyses of single cells we identify asymmetric cell volume changes along the anteroposterior axis of KV that coincide with asymmetric cell shape changes. Blocking ion flux prevents these cell volume changes and cell shape changes. Vertex simulations suggest cell shape changes do not depend on lumen expansion. Consistent with this prediction, asymmetric changes in KV cell volume and shape occur normally when KV lumen growth fails due to leaky cell adhesions. These results indicate ion flux mediates cell volume changes that contribute to asymmetric cell shape changes in KV, and that these changes in epithelial morphology are separable from lumen-generated forces.