serial time-encoded amplified microscopy for high-speed contrast-enhanced imaging of transparent media.
serial time-encoded amplified microscopy for high-speed contrast-enhanced imaging of transparent media.
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发表时间：
2011
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通讯作者：
A. Fard;A. Mahjoubfar;K. Goda;D. Gossett;D. Di Carlo;B. Jalali
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作者：
A. Fard;A. Mahjoubfar;K. Goda;D. Gossett;D. Di Carlo;B. Jalali
：高速高对比度成像模式能够在不需要化学染色的情况下获取透明介质的图像，是从半导体工艺监控到血液筛查等广泛应用的基本工具。在这里，我们介绍了一种对比度增强成像的未染色的透明物体，能够高通量成像的方法。该方法将Nomarski相衬能力与串行时间编码放大显微镜的超高帧速率和快门速度相结合。作为概念的证明，我们使用单像素光电探测器以33 ps的快门速度和36.1 MHz的帧速率显示了透明测试结构和流动中的白色血细胞的成像。该方法有望成为高通量筛选未染色细胞的一种有价值的工具。
: High-speed high-contrast imaging modalities that enable image acquisition of transparent media without the need for chemical staining are essential tools for a broad range of applications; from semiconductor process monitoring to blood screening. Here we introduce a method for contrast-enhanced imaging of unstained transparent objects that is capable of high-throughput imaging. This method combines the Nomarski phase contrast capability with the ultrahigh frame rate and shutter speed of serial time-encoded amplified microscopy. As a proof of concept, we show imaging of a transparent test structure and white blood cells in flow at a shutter speed of 33 ps and a frame rate of 36.1 MHz using a single-pixel photo-detector. This method is expected to be a valuable tool for high-throughput screening of unstained cells.