A systematic study on hemocyte identification and plasma prophenoloxidase from Culex pipiens quinquefasciatus at different developmental stages
A systematic study on hemocyte identification and plasma prophenoloxidase from Culex pipiens quinquefasciatus at different developmental stages
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致倦库蚊不同发育阶段血细胞鉴定及血浆酚氧化酶原的系统研究
DOI：
10.1016/j.exppara.2010.07.005
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发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Ling, Erjun
中科院分区：
文献类型：
--
作者：
Wang, Zhixiang;Lu, Anrui;Ling, Erjun
致倦库蚊Culex pipiens quinquefasciatus（C.致倦丝绦虫（quinquefasciatus）是传播西尼罗河病毒、淋巴丝虫病、日本脑炎和圣路易斯脑炎等人类疾病的重要媒介。然而，关于致倦库蚊的体液和细胞免疫防御的研究非常有限。本文介绍了红曲霉血细胞的鉴定和血浆中含血细胞酚氧化酶原的研究。为了获得致倦库蚊C.致倦犬先天免疫我们将血细胞分为四种类型：原血细胞、类卵细胞、浆细胞和粒细胞。酚氧化酶原（Prophenoloxidase，PPO）是微胶囊化后引起色素黑化的重要酶。PPO阳性血细胞和血浆PPO观察在所有的发展阶段。至于特定的血细胞类型，酚氧化酶原被发现在浆细胞在幼虫阶段单独和最小的原血细胞在几乎所有的发展阶段。吸血雌蚊的粒细胞呈PPO阳性，吸血后蛹和成年雌蚊的类卵细胞呈PPO阳性。而血浆中PPO的含量则不同。quinquefasciatus在不同的发展阶段。这些结果为进一步研究C. quinquefasciatus血细胞和酚氧化酶原以及它们参与对抗蚊媒病原体。(C)2010年爱思唯尔公司All rights reserved.
Culex pipiens quinquefasciatus (C. quinquefasciatus) is an important vector that can transmit human diseases such as West Nile virus, lymphatic filariasis, Japanese encephalitis and St. Louis encephalitis. However, very limited research concerning the humoral and cellular immune defenses of C quinquefasciatus has been done. Here we present the research on hemocyte identification and plasma including hemocyte prophenoloxidase from C. quinquefasciatus at all developmental stages in order to obtain a complete picture of C. quinquefasciatus innate immunity. We identified hemocytes into four types: prohemocytes, oenocytoids, plasmatocytes and granulocytes. Prophenoloxidase (PPO) is an essential enzyme to induce melanization after encapsulation. PPO-positive hemocytes and plasma PPO were observed at all developmental stages. As for specific hemocyte types, prophenoloxidase was found in the plasmatocytes at larval stage alone and in the smallest prohemocytes during almost all developmental stages. Moreover, the granulocytes were PPO-positive from blood-fed female mosquitoes and oenocytoids were observed PPO-positive in pupae and in adult females after blood-feeding. As for plasma, there were different patterns of PPO in C. quinquefasciatus at different developmental stages. These results are forming a basis for further studies on the function of C. quinquefasciatus hemocytes and prophenoloxidase as well as their involvement in fighting against mosquito-borne pathogens. (C) 2010 Elsevier Inc. All rights reserved.