Deciphering the roles of circRNAs on chilling injury in tomato.
Deciphering the roles of circRNAs on chilling injury in tomato.
复制标题
DOI：
10.1016/j.bbrc.2016.07.032
复制
发表时间：
2016-10
影响因子：
3.1
通讯作者：
Jinhua Zuo;Qing Wang;B. Zhu;Yunbo Luo;Lipu Gao
中科院分区：
文献类型：
--
作者：
Jinhua Zuo;Qing Wang;B. Zhu;Yunbo Luo;Lipu Gao
近年来，通过高通量深度测序，在动植物中发现了许多新的环状RNA（circular RNA，circRNA），它们在miRNA的功能和转录调控中发挥着重要作用。然而，对番茄果实中的circRNA知之甚少。本研究采用深度测序和生物信息学相结合的方法，系统解析了全基因组中的circRNA。共鉴定出854个circRNA，其中163个circRNA具有低温响应性表达。研究表明，一些circRNA参与了植物的低温响应过程，如氧化还原反应、细胞壁降解、精氨酸和多胺代谢、热激和冷激蛋白、能量代谢、茉莉酸和脱落酸代谢、低温和盐响应蛋白以及低温诱导转录因子（CBF和WRKY）等。此外，在番茄中还发现了102个circRNA作为相应的24个miRNAs海绵。这些发现揭示了调控circRNA的意想不到的复杂性，并可能为理解植物中circRNA的功能打开一扇窗户。
Various circular RNAs (circRNAs) are newly identified in animals and plants through high-throughput deep sequencing which play important roles in miRNA function and transcriptional controlling. However, little is known regarding circRNAs in tomato fruit. In this study, we systematically parse the circRNAs in the whole genome using the combination methods of deep sequencing and bioinformatics. In all, 854 circRNAs were identified in our results, among them, 163 circRNAs exhibit chilling responsive expression. Intriguingly, several circRNAs were predicted to involved in chilling responsive process, such as redox reaction, cell wall degradation, Arginine and polyamine metabolism, heat and cold shock protein, energy metabolism, Jasmonic acid and abscisic acid metabolism, low temperature and salt responsive protein and low temperature-induced transcription factors(CBF and WRKY). Furthermore, 102 circRNAs were found to act as the corresponding 24 miRNAs sponges in tomato. These discoveries deciphered the unexpected complexity of the regulatory circRNAs and may open a window for understanding the functions of circRNAs in plants.