miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice
miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice
复制标题
miR-34b-3p 抑制 eIF4E 导致成年小鼠中风后抑郁
DOI：
10.1007/s12264-022-00898-7
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Lei Pei
中科院分区：
文献类型：
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作者：
Xiao Ke;Manfei Deng;Zhuoze Wu;Hongyan Yu;Dian Yu;Hao Li;Youming Lu;Kai Shu;Lei Pei
Post-stroke depression (PSD) is a serious and common complication of stroke, which seriously affects the rehabilitation of stroke patients. To date, the pathogenesis of PSD is unclear and effective treatments remain unavailable. Here, we established a mouse model of PSD through photothrombosis-induced focal ischemia. By using a combination of brain imaging, transcriptome sequencing, and bioinformatics analysis, we found that the hippocampus of PSD mice had a significantly lower metabolic level than other brain regions. RNA sequencing revealed a significant reduction of miR34b-3p, which was expressed in hippocampal neurons and inhibited the translation of eukaryotic translation initiation factor 4E (eIF4E). Furthermore, silencing eIF4E inactivated microglia, inhibited neuroinflammation, and abolished the depression-like behaviors in PSD mice. Together, our data demonstrated that insufficient miR34b-3p after stroke cannot inhibit eIF4E translation, which causes PSD by the activation of microglia in the hippocampus. Therefore, miR34b-3p and eIF4E may serve as potential therapeutic targets for the treatment of PSD.
