Long non-coding RNA expression profiling of macrophage line RAW264.7 infected by Mycobacterium tuberculosis
Long non-coding RNA expression profiling of macrophage line RAW264.7 infected by Mycobacterium tuberculosis
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结核分枝杆菌感染的巨噬细胞系RAW264.7的长非编码RNA表达谱
DOI：
10.1080/10520295.2019.1707874
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发表时间：
2020-02-21
影响因子：
1.6
通讯作者：
Li, Junming
中科院分区：
文献类型：
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作者：
Huang, Zikun;Liu, Jianing;Li, Junming
摘要长非编码RNA(LncRNAs)参与了生物过程的调控。巨噬细胞中的lncRNAs在结核分枝杆菌感染反应中的作用尚未被探索。我们使用高通量的lncRNA芯片分析来检测在有或没有结核分枝杆菌感染的RAW264.7巨噬细胞中差异表达的lncRNAs和mRNAs。采用实时定量聚合酶链式反应(qRT-PCR)对芯片结果进行验证。生物信息学分析(GO和KEGG)用于探索显著异常基因的功能。基因芯片结果显示，与对照组相比，结核分枝杆菌感染的RAW264.7巨噬细胞有1,487个lncRNAs(791个上调和696个下调)和910个mRNAs(536个上调和374个下调)表达差异。GO和通路分析表明，上调的mRNAs参与了免疫反应、免疫系统过程、系统发育或肿瘤坏死因子信号通路，以及抗原的处理和提呈。相反，下调的mRNAs参与了系统的发育、生物学过程的调节和PPAR信号通路。10个LncRNAs和mRNAs的QRT-PCR结果与芯片数据一致。结核分枝杆菌感染巨噬细胞后，LncRNA AK151345的表达增强，且呈时间和剂量依赖性。我们确定了在结核分枝杆菌感染的RAW264.7巨噬细胞中差异表达的lncRNAs的综合表达谱。
ABSTRACT Long non-coding RNAs (lncRNAs) have been implicated in regulation of biological processes. The role of lncRNAs in macrophages in response to Mycobacterium tuberculosis infection has not been explored. We used high throughput lncRNA microarray analysis to detect differentially expressed lncRNAs and mRNAs in RAW264.7 macrophages with or without M. tuberculosis infection. Quantitative real-time PCR (qRT-PCR) was used to verify the microarray results. Bioinformatics analysis (GO and KEGG) were used to explore the function of significantly dysregulated genes. Microarray results indicated that 1,487 lncRNAs (791 up and 696 down) and 910 mRNAs (536 up and 374 down) were expressed differentially in RAW264.7 macrophages with M. tuberculosis infection compared to controls. GO and pathway analysis revealed that up-regulated mRNAs were involved in immune response, immune system process, system development or TNF signaling pathway, and antigen processing and presentation. To the contrary, down-regulated mRNAs participated in system development, regulation of biological processes and peroxisome proliferator-activated receptor (PPAR) signaling pathway. qRT-PCR results of 10 lncRNAs and mRNAs were consistent with the microarray data. M. tuberculosis infection of macrophages caused enhanced expression of lncRNA AK151345 in a time- and dose-dependent manner. We determined comprehensive expression profiles of differentially expressed lncRNAs in RAW264.7 macrophages infected by M. tuberculosis.