A transcriptomic analysis of the phylum Nematoda
A transcriptomic analysis of the phylum Nematoda
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DOI：
10.1038/ng1472
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发表时间：
2004-12-01
期刊：
影响因子：
30.8
通讯作者：
Blaxter, ML
中科院分区：
文献类型：
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作者：
Parkinson, J;Mitreva, M;Blaxter, ML
线虫动物门占据了一个巨大的生态位范围，从自由生活的微生物到人类寄生虫。我们使用265，494个表达序列标签序列分析了该门的基因组生物学，对应于来自30个物种（包括28种寄生虫）的93，645个推定基因。每个物种的35%至70%的基因与模式线虫秀丽隐杆线虫的蛋白质具有显著的相似性。超过一半的推定基因是该门所特有的，23%是它们所来源的物种所特有的。我们还没有接近耗尽该门的基因组多样性。我们确定了超过2,600种不同的已知蛋白质结构域，其中一些在线虫的主要分类群之间具有差异丰度。我们还从线虫限制性基因中定义了4，228个线虫特异性蛋白质家族：这类基因可能是物种和更高级别分类学差异的基础。线虫特异性家族作为药物和疫苗靶标特别令人感兴趣。
The phylum Nematoda occupies a huge range of ecological niches, from free-living microbivores to human parasites. We analyzed the genomic biology of the phylum using 265,494 expressed-sequence tag sequences, corresponding to 93,645 putative genes, from 30 species, including 28 parasites. From 35% to 70% of each species' genes had significant similarity to proteins from the model nematode Caenorhabditis elegans. More than half of the putative genes were unique to the phylum, and 23% were unique to the species from which they were derived. We have not yet come close to exhausting the genomic diversity of the phylum. We identified more than 2,600 different known protein domains, some of which had differential abundances between major taxonomic groups of nematodes. We also defined 4,228 nematode-specific protein families from nematode-restricted genes: this class of genes probably underpins species- and higher-level taxonomic disparity. Nematode-specific families are particularly interesting as drug and vaccine targets.