A BROAD-SPECTRUM ARTIFICIAL SEAWATER MEDIUM FOR COASTAL AND OPEN OCEAN PHYTOPLANKTON
A BROAD-SPECTRUM ARTIFICIAL SEAWATER MEDIUM FOR COASTAL AND OPEN OCEAN PHYTOPLANKTON
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DOI：
10.1111/j.1529-8817.1980.tb02994.x
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发表时间：
1980-01-01
影响因子：
2.9
通讯作者：
TAYLOR, FJR
中科院分区：
文献类型：
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作者：
HARRISON, PJ;WATERS, RE;TAYLOR, FJR
用来自11个不同藻类的83株近海和远洋浮游植物对一种新的人工海水培养基进行了试验。培养进行了4次转移，对大多数物种来说，周期为8wk。只有3种藻类不能在富营养化的人工海水中存活，而16种藻类的最终细胞产量比在富营养化的天然海水中生长的藻类有所下降，其中主要来自裸藻和裸藻。由于77%的受测物种在浓缩的人工或天然海水中生长得同样好，超过95%的物种可以在人工介质中保持生长，因此这个配方在广泛的物种范围内都很有用。用改进的富集液对人工海水碱基进行富集化；主要的修改是省略Tris和添加Si。在高压灭菌前，通过降低pH值，在不沉淀的情况下对浓缩介质进行高压灭菌。这是通过加入等摩尔量的Na-HCO3和HCl来实现的，在加热过程中会产生氯化钠和二氧化碳。当不使用pH缓冲液时，只有将人工海水基质高压灭菌为两种不同的盐溶液(从CO3-2和SO4-2中分离出钙和锶)，冷却、混合并无菌加入灭菌富集液，才能避免沉淀。
A new artificial seawater medium was tested with 83 strains of coastal and open ocean phytoplankton from 11 different algal classes. The cultures were carried through 4 transfers, representing a period of 8 wk for most species. Only 3 species could not be maintained in the enriched artificial seawater, and 16 spp., mainly from the Prymnesiophyceae and Dinophyceae, had reduced final cell yields compared to those grown in enriched natural seawater. Since 77% of the species tested grew equally well in enriched artificial or natural seawater and more than 95% could be maintained in the artificial medium, this recipe is useful over a broad spectrum of species. The artificial seawater base was enriched with a modified enrichment solution; the primary modifications were the omission of Tris and the addition of Si. Enriched medium was autoclaved without precipitation by lowering the pH before autoclaving. This was accomplished by adding equimolar amounts of Na-HCO3 and HCl which produced NaCl and CO2 during the heating process. When no pH buffer was used, precipitation could only be avoided by autoclaving the artificial seawater base as 2 separate salt solutions (with Ca and Sr separated from CO3-2 and SO4-2), cooling, mixing and aseptically adding the sterilized enrichment solution.