A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection
A universal label-free fluorescent aptasensor based on Ru complex and quantum dots for adenosine, dopamine and 17β-estradiol detection
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DOI：
10.1016/j.bios.2015.12.024
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发表时间：
2016-05-15
影响因子：
12.6
通讯作者：
Yao, Tianming
中科院分区：
文献类型：
--
作者：
Huang, Hailiang;Shi, Shuo;Yao, Tianming
基于特异性的适配体结合特性，采用钌配合物和量子点（QD）作为荧光探针，实现了腺苷，多巴胺和17 β-雌二醇的检测策略。钌配合物对量子点的荧光有猝灭作用，并优先与核酸适体结合，导致量子点的荧光增强。当适体DNA先与靶孵育时，它不能与钌配合物结合，量子点的荧光被淬灭。在最佳条件下，腺苷、多巴胺和17 β-磷脂酰胆碱的浓度与荧光强度呈线性关系，检测限分别为101 nM、19 nM和37 nM。在胎牛血清中也进行了实验，结果良好。该方法具有快速、免标记、成本低、操作简便、重复性好等优点，可用于腺苷、多巴胺和17 β-雌二醇的检测。肉眼定性检测也是可行的，无需复杂的仪器。它也可以扩展到检测各种分析物，如金属离子，蛋白质和小分子，通过使用适当的适配体。(C)2015爱思唯尔B. V.保留所有权利。
Based on specific aptamer binding properties, a strategy for adenosine, dopamine and 17 beta-estradiol detection was realised by employing Ru complex and quantum dots (QDs) as fluorescence probes. Ru complex, which could quench the fluorescence of QDs, preferred to bind with aptamer DNA and resulted in the fluorescence rise of QDs. When the aptamer DNA was incubated with the target first, it could not bind with Ru complex and the fluorescence of QDs was quenched. Under the optimal condition, the fluorescence intensity was linearly proportional to the concentration of adenosine, dopamine and 17 beta-Pestradiol with a limit of detection (LOD) of 101 nM, 19 nM and 37 nM, respectively. The experiments in fetal bovine serum were also carried out with good results. This universal method was rapid, label-free, low-cost, easy-operating and highly repeatable for the detection of adenosine, dopamine and 17 beta-estradiol. Qualitative detection by naked eyes was also available without complex instruments. It could also be extended to detect various analytes, such as metal ions, proteins and small molecules by using appropriate aptamers. (C) 2015 Elsevier B.V. All rights reserved.