Engineering and identifying supercharged proteins for macromolecule delivery into mammalian cells.
Engineering and identifying supercharged proteins for macromolecule delivery into mammalian cells.
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DOI：
10.1016/b978-0-12-396962-0.00012-4
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发表时间：
2012
影响因子：
--
通讯作者：
Liu, David R.
中科院分区：
文献类型：
--
作者：
Thompson, David B.;Cronican, James J.;Liu, David R.
超电荷蛋白质是一类工程或天然存在的蛋白质，具有异常高的净正或负理论电荷。带超负电荷和带超正电荷的蛋白质都表现出显著的耐受热或化学诱导的聚集的能力。带超级电荷的蛋白质也能够穿透哺乳动物细胞。将货物与这些蛋白质（例如质粒DNA、siRNA或其他蛋白质）缔合可以使这些大分子能够在体外和体内功能性递送到哺乳动物细胞中。在某些情况下，功能性递送的效力可以超过用于大分子递送的其它当前方法的效力，包括使用细胞穿透肽如达特和腺病毒递送载体。本章总结了工程增压蛋白质，优化细胞渗透，识别天然存在的增压蛋白质，并使用这些蛋白质的大分子传递到哺乳动物细胞的方法。
Supercharged proteins are a class of engineered or naturally occurring proteins with unusually high net positive or negative theoretical charge. Both supernegatively and superpositively charged proteins exhibit a remarkable ability to withstand thermally or chemically induced aggregation. Superpositively charged proteins are also able to penetrate mammalian cells. Associating cargo with these proteins, such as plasmid DNA, siRNA, or other proteins, can enable the functional delivery of these macromolecules into mammalian cells both in vitro and in vivo. The potency of functional delivery in some cases can exceed that of other current methods for macromolecule delivery, including the use of cell-penetrating peptides such as Tat, and adenoviral delivery vectors. This chapter summarizes methods for engineering supercharged proteins, optimizing cell penetration, identifying naturally occurring supercharged proteins, and using these proteins for macromolecule delivery into mammalian cells.