Principal Neurons in the Anteroventral Cochlear Nucleus Express Cell-Type Specific Glycine Receptor α Subunits
Principal Neurons in the Anteroventral Cochlear Nucleus Express Cell-Type Specific Glycine Receptor α Subunits
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DOI：
10.1016/j.neuroscience.2019.07.019
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发表时间：
2019-09-01
期刊：
影响因子：
3.3
通讯作者：
Xie, Ruili
中科院分区：
文献类型：
--
作者：
Lin, Shengyin;Xie, Ruili
耳蜗前腹侧核（AVCN）主要神经元的信号处理受到甘氨酸能抑制的调节。IPSC的动力学对靶神经元是特异性的。目前还不清楚什么样的甘氨酸受体亚基参与AVCN主神经元中产生这种靶特异性IPSC动力学。我们用免疫组织化学方法标记甘氨酸受体α亚基的四种亚型（GlyR α（1）-α（4）），研究了AVCN中GlyR α亚基的表达模式，发现AVCN神经元表达GlyR α（1）和GlyR α（4），但不表达GlyR α（2）和GlyR α（3）亚基。为了进一步鉴定GlyR α亚基的细胞类型特异性表达模式，我们将全细胞膜片钳记录与免疫组织化学相结合，通过记录所有三种类型的AVCN主要神经元，表征其甘氨酸能抑制的突触特性，染料填充神经元，并处理切片用于不同GlyR α亚基的免疫染色。我们发现AVCN丛状神经元表达GlyR α（1）和GlyR α（4）亚基，这是其慢IPSC动力学的基础，而T-星状神经元和D-星状神经元仅表达GlyR α（1）亚基，这是其快IPSC动力学的基础。总之，AVCN主要神经元表达细胞类型特异性GlyR α亚基，这是其独特的IPSC动力学的基础，这使得来自相同来源的甘氨酸能抑制能够发挥靶细胞特异性活性调节，以支持这些神经元的独特生理功能。(C)2019年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Signal processing in the principal neurons of the anteroventral cochlear nucleus (AVCN) is modulated by glycinergic inhibition. The kinetics of IPSCs are specific to the target neurons. It remains unclear what glycine receptor subunits are involved in generating such target-specific IPSC kinetics in AVCN principal neurons. We investigated the expression patterns of glycine receptor alpha (GlyR alpha) subunits in AVCN using immunohistochemical labeling of four isoforms of GlyR alpha subunits (GlyR alpha(1)-alpha(4)), and found that AVCN neurons express GlyR alpha(1) and GlyR alpha(4), but not GlyR alpha(2) and GlyR alpha(3) subunits. To further identify the cell type-specific expression patterns of GlyR alpha subunits, we combined whole-cell patch clamp recording with immunohistochemistry by recording from all three types of AVCN principal neurons, characterizing the synaptic properties of their glycinergic inhibition, dye-filling the neurons, and processing the slice for immunostaining of different GlyR alpha subunits. We found that AVCN bushy neurons express both GlyR alpha(1) and GlyR alpha(4) subunits that underlie their slow IPSC kinetics, whereas both T-stellate and D-stellate neurons express only GlyR alpha(1) subunit that underlies their fast IPSC kinetics. In conclusion, AVCN principal neurons express cell-type specific GlyR alpha subunits that underlie their distinct IPSC kinetics, which enables glycinergic inhibition from the same source to exert target cell-specific modulation of activity to support the unique physiological function of these neurons. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.