Inhibition of hepatocyte growth factor-induced motility and in vitro invasion of human colon cancer cells by gamma-linolenic acid.
Inhibition of hepatocyte growth factor-induced motility and in vitro invasion of human colon cancer cells by gamma-linolenic acid.
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DOI：
10.1038/bjc.1995.145
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发表时间：
1995-04
影响因子：
8.8
通讯作者：
Puntis, M C
中科院分区：
文献类型：
--
作者：
Jiang, W G;Hiscox, S;Hallett, M B;Scott, C;Horrobin, D F;Puntis, M C
在这项研究中，我们确定了 n-6 必需脂肪酸 γ-亚麻酸 (GLA) 对人类结肠癌细胞系 HT115、HT29 和 HRT18 的运动性和侵袭性/转移性的影响。细胞运动由肝细胞生长因子/散射因子 (HGF/SF) 诱导，并通过集落散射和载体珠解离来测量。通过细胞侵入细胞外基质在体外测量侵袭性。在浓度高达 100 microM 时（在实验期间对细胞生长没有影响），GLA 及其锂盐显着降低了 HGF/SF 诱导的细胞运动和侵袭。这些细胞与细胞外基质成分（基质胶和纤连蛋白）的附着也受到抑制。细胞表面 E-钙粘蛋白也发生变化，但在相似浓度下纤连蛋白受体没有变化。结论是，n-6 必需脂肪酸可能通过修饰细胞表面粘附分子来抑制人结肠癌细胞的运动性和侵袭性。
In this study we have determined the effects of the n-6 essential fatty acid gamma-linolenic acid (GLA) on the motility and invasive/metastatic nature of the human colon cancer cell lines HT115, HT29 and HRT18. Cell motility was induced by hepatocyte growth factor/scatter factor (HGF/SF) and measured by both colony scattering and dissociation from carrier beads. Invasiveness was measured in vitro by cellular invasion into extracellular matrix. At concentrations up to 100 microM (which had no effect on cell growth over the duration of the experiments) both cell motility and invasion induced by HGF/SF were markedly reduced by GLA and its lithium salt. The attachment of these cells to the extracellular matrix components (Matrigel and fibronectin) was also inhibited. There were also changes in the cell-surface E-cadherin, but not fibronectin receptor at similar concentrations. It is concluded that n-6 essential fatty acids have the ability to inhibit both motility and invasiveness of human colon cancer cells, perhaps by modifying cell-surface adhesion molecules.