Identification of Three Prolactin-Related Hormones as Markers of Invasive Trophoblasts in the Rat1
Identification of Three Prolactin-Related Hormones as Markers of Invasive Trophoblasts in the Rat1
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DOI：
10.1095/biolreprod63.2.519
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发表时间：
2000-08
期刊：
影响因子：
--
通讯作者：
D. Toft;D. Linzer
中科院分区：
文献类型：
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作者：
D. Toft;D. Linzer
摘要表达序列标签数据库搜索已经确定了三个大鼠的cDNA克隆在催乳素/生长激素家族，包括小鼠增殖相关蛋白（PRP）的同源物。这两个新克隆的编码蛋白质，命名为催乳素样蛋白L（PLP-L）和M（PLP-M），被预测合成为229和227个氨基酸的前体，通过N-连接糖基化修饰，并分别分泌为199和200个残基的成熟糖蛋白。还鉴定了PLP-L和PLP-M的鼠同系物。大鼠PRP的开放阅读框架编码245个氨基酸的前体蛋白，并预测与小鼠PRP具有81%同一性的分泌的215个氨基酸的糖蛋白。所有三种大鼠mRNA都在胎盘中表达，在其他组织中未检测到表达。从第11-20天观察到PLP-L mRNA表达，在第13天达到最高水平;从第11天至分娩观察到PLP-M的最高水平，也在第13天达到峰值水平;从第11天至足月也观察到PRP的最高水平，在第17天达到最大表达。所有这三种基因在中央胎盘血管内层的浸润性滋养层细胞中表达最高。血管内滋养层细胞的分子标志物的鉴定有助于突出啮齿动物胎盘形成的侵入性，并可能对未来胎盘功能的研究有用。
Abstract An expressed-sequence tag database search has identified three rat cDNA clones in the prolactin/growth hormone family, including a homologue of mouse proliferin-related protein (PRP). The encoded proteins of the two novel clones, designated prolactin-like proteins L (PLP-L) and M (PLP-M), are predicted to be synthesized as precursors of 229 and 227 amino acids, modified by N-linked glycosylation, and secreted as mature glycoproteins of 199 and 200 residues, respectively. Murine homologues to PLP-L and PLP-M were also identified. The open reading frame of rat PRP encodes a precursor protein of 245 amino acids and predicts a secreted 215-amino acid glycoprotein with 81% identity to mouse PRP. All three rat mRNAs are expressed in the placenta, and expression is not detected in other tissues. PLP-L mRNA expression is observed from Days 11–20, with highest levels at Day 13; highest levels of PLP-M are observed from Day 11 until parturition, with peak levels also on Day 13; and highest levels of PRP are also observed from Day 11 until term, with maximal expression on Day 17. All three genes are most highly expressed in invasive trophoblast cells lining the central placental vessel. The identification of molecular markers for endovascular trophoblasts serves to highlight the invasive nature of rodent placentation and may prove useful for future studies of placental function.