Rules for gene usage inferred from a comparison of large-scale gene expression profiles of T and B lymphocyte development
Rules for gene usage inferred from a comparison of large-scale gene expression profiles of T and B lymphocyte development
复制标题
DOI：
10.4049/jimmunol.170.3.1339
复制
发表时间：
2003-02-01
影响因子：
4.4
通讯作者：
Melchers, F
中科院分区：
文献类型：
--
作者：
Hoffmann, R;Bruno, L;Melchers, F
在高密度寡核苷酸阵列上产生小鼠胸腺细胞发育的七个连续阶段的核糖核酸表达谱。证实了先前已知的几个基因的表达模式。10%（13,000多个基因中的1,304个）被发现在所有T细胞发育阶段有99%的差异表达。当与在骨髓的五个连续B谱系发育阶段中差异表达的1，204个基因相比时，>40%（546个基因）似乎被两个谱系共享。然而，当在B和T细胞发育之间比较四个功能不同的细胞阶段时，DJ重排的前体细胞和静息的未成熟前体细胞在表面Ag受体表达之前和之后共享不到10%，成熟的静息淋巴细胞在15%和20%之间，并且仅响应于前体淋巴细胞受体沉积的循环前体共享>50%的这些差异表达基因。从这些结果中出现三个一般规则：1）在可比较阶段的细胞增殖大多数由相同的基因执行; 2）细胞内信号传导和细胞间通讯主要受不同基因的影响; 3）大多数基因不是严格地在可比较的阶段使用，而是在几个阶段使用，可能在不同的功能环境中。
Ribonucleic acid expression profiles of seven consecutive stages of mouse thymocyte development were generated on high density oligonucleotide arrays. Previously known expression patterns of several genes were confirmed. Ten percent (1,304 of more than 13,000) of the monitored genes were found with 99% confidence to be differentially expressed across all T cell developmental stages. When compared with 1,204 genes differentially expressed in five consecutive B lineage developmental stages of bone marrow, >40% (546 genes) appeared to be shared by both lineages. However, when four pools of functionally distinct cell stages were compared between B and T cell development, DJ-rearranged precursor cells and resting immature precursor cells before and after surface Ag receptor expression shared less than 10%, mature resting lymphocytes between 15 and 20%, and only cycling precursors responding to precursor lymphocyte receptor deposition shared >50% of these differentially expressed genes. Three general rules emerge from these results: 1) proliferation of cells at comparable stages is in majority executed by the same genes; 2) intracellular signaling and intercellular communication are effected largely by different genes; and 3) most genes are not used strictly at comparable, but rather at several, stages, possibly in different functional contexts.