Comparative analysis of bacterial community and antibiotic-resistant strains in different developmental stages of the housefly (Musca domestica)
Comparative analysis of bacterial community and antibiotic-resistant strains in different developmental stages of the housefly (Musca domestica)
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DOI：
10.1007/s00253-012-4024-1
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发表时间：
2012-04
影响因子：
5
通讯作者：
Tingting Wei;Jun Hu;K. Miyanaga;Y. Tanji
中科院分区：
文献类型：
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作者：
Tingting Wei;Jun Hu;K. Miyanaga;Y. Tanji
家蝇是多种细菌的重要宿主，包括一些致病性和抗药性菌株。为了进一步研究家蝇和它所携带的细菌之间的关系，有必要了解幼虫变态过程中微生物的命运。通过非培养方法，16 S rRNA基因的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析，研究了家蝇三个发育阶段（蛆、蛹和成虫）的主要细菌群落。利用DGGE分析鉴定的细菌跨越变形菌门、厚壁菌门和拟杆菌门。在家蝇发育过程中，优势菌属发生了变化，蛆中检出拟杆菌属、库口氏菌属和Schineria，蛹中检出奈瑟氏菌属，成蝇中检出巨球菌属、乳球菌属和库尔西氏菌属。使用选择性培养基筛选抗生素耐药菌，并测试抗生素敏感性。从蛆和蛹中分离的大多数抗性菌株被归类为变形杆菌属，而来自成年蝇的则更加多样化，跨越了12个属。在三个阶段的20个测试菌株中，18个对至少两种抗生素具有耐药性。总体而言，我们证明了随着家蝇的发展，主要细菌群落和抗药性菌株发生了变化。
The housefly (Musca domestica) is an important host for a variety of bacteria, including some pathogenic and antibiotic-resistant strains. To further investigate the relationship between the housefly and the bacteria it harbors, it is necessary to understand the fate of microorganisms during the larval metamorphosis. The major bacterial communities in three developmental stages of the housefly (maggot, pupa, and adult fly) were investigated by a culture-independent method, polymerase chain reaction–denaturing gradient gel electrophoresis (PCR−DGGE) analysis of 16S rRNA genes. The bacteria that were identified using DGGE analysis spanned phylaProteobacteria,Firmicutes, andBacteroidetes. Changes in the predominant genera were observed during the housefly development.Bacteroides,Koukoulia, andSchineriawere detected in maggots,Neisseriain pupae, andMacrococcus,Lactococcus, andKurthiain adult flies. Antibiotic-resistant bacteria were screened using a selective medium and tested for antibiotic susceptibility. Most resistant isolates from maggots and pupae were classified asProteusspp., while those from adult flies were much more diverse and spanned 12 genera. Among 20 tested strains across the three stages, 18 were resistant to at least two antibiotics. Overall, we demonstrated that there are changes in the major bacterial communities and antibiotic-resistant strains as the housefly develops.