Tongue Fat Infiltration in Obese Versus Lean Zucker Rats
Tongue Fat Infiltration in Obese Versus Lean Zucker Rats
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DOI：
10.5665/sleep.3768
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发表时间：
2014-06-01
期刊：
影响因子：
5.6
通讯作者：
Schwab, Richard J.
中科院分区：
文献类型：
--
作者：
Brennick, Michael J.;Delikatny, James;Schwab, Richard J.
研究目的：肥胖是阻塞性睡眠呼吸暂停（OSA）最重要的危险因素，而肥胖的影响可能与舌脂介导有关。我们的目的是研究肥胖对肥胖（OBZ）和非肥胖（NBZ） Zucker大鼠上呼吸道结构的影响。设计：动物实验。环境：学术医疗中心。参与者：OBZ （638.2 +/- 39 g; 14.9 +/- 1.1 w）和年龄匹配的NBZ Zucker （442.6 +/- 37 g, 15.1 +/- 1.5 w）大鼠。干预措施：使用体内磁共振波谱（MRS）、磁共振成像（包括Dixon成像）评估舌脂肪体积、离体生物化学(脂肪定量、甘油三酯(mg)/组织(g)和组织学（油红O染色）评估舌脂肪和体积。测量和结果：MRS：整体OBZ舌脂/水比为NBZ的2.9倍（P < 0.002），其中前OBZ舌脂/水比为NBZ的3.3倍（P < 0.002）。生物化学：舌内甘油三酯（TG）在OBZ组比在NBZ组高4.4倍(P
Study Objectives: Obesity is the most important risk factor for obstructive sleep apnea (OSA), and the effects of obesity may be mediated by tongue fat. Our objective was to examine the effects of obesity on upper airway structures in obese (OBZ) and non-obese (NBZ) Zucker rats.Design: Animal study.Setting: Academic Medical Center.Participants: OBZ (638.2 +/- 39 g; 14.9 +/- 1.1 w) and age-matched NBZ Zucker (442.6 +/- 37 g, 15.1 +/- 1.5 w) rats.Interventions: Tongue fat and volume and were assessed using: in vivo magnetic resonance spectroscopy (MRS), magnetic resonance imaging including Dixon imaging for tongue fat volume, ex vivo biochemistry (fat quantification; triglyceride (mg)/tissue (g), and histology (Oil Red O stain).Measurements and Results: MRS: overall OBZ tongue fat/water ratio was 2.9 times greater than NBZ (P < 0.002) with the anterior OBZ tongue up to 3.3 times greater than NBZ (P < 0.002). Biochemistry: Triglyceride (TG) in the tongue was 4.4 times greater in OBZ versus NBZ (P