A 5' ELEMENT OF THE CHICKEN BETA-GLOBIN DOMAIN SERVES AS AN INSULATOR IN HUMAN ERYTHROID-CELLS AND PROTECTS AGAINST POSITION EFFECT IN DROSOPHILA
A 5' ELEMENT OF THE CHICKEN BETA-GLOBIN DOMAIN SERVES AS AN INSULATOR IN HUMAN ERYTHROID-CELLS AND PROTECTS AGAINST POSITION EFFECT IN DROSOPHILA
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DOI：
10.1016/0092-8674(93)80052-g
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发表时间：
1993-08-13
期刊：
影响因子：
64.5
通讯作者：
FELSENFELD, G
中科院分区：
文献类型：
--
作者：
CHUNG, JH;WHITELEY, M;FELSENFELD, G
我们已经表征了鸡β-珠蛋白结构域的5'边界附近的元件，当在人红系细胞系K562中测定时，该元件将报告基因与附近的β-珠蛋白基因座控制区（5' HS 2）的激活效应隔离。我们表明，绝缘机制是定向的，它在转录水平上运作，它涉及到基因启动子上染色质结构的改变。绝缘体本身没有明显的刺激或抑制作用。在转基因果蝇中，绝缘子保护白色小基因免受位置效应的影响。因此，绝缘子的作用并不局限于红细胞或哺乳动物细胞，这表明这些元件可能在染色质结构的组织中发挥重要且广泛分布的功能。
We have characterized an element near the 5' boundary of the chicken beta-globin domain that insulates a reporter gene from the activating effects of a nearby beta-globin locus control region (5'HS2) when assayed in the human erythroid cell line K562. We show that the insulation mechanism is directional, that it operates at the level of transcription, and that it involves the alteration of chromatin structure over the promoter of the gene. The insulator has no significant stimulatory or inhibitory effects of its own. In transgenic Drosophila, the insulator protects the white minigene from position effects. The action of the insulator thus is not restricted to erythroid or mammalian cells, suggesting that such elements may serve an important and widely distributed function in the organization of chromatin structure.