A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES
A SYSTEMATIC NOMENCLATURE FOR CARBOHYDRATE FRAGMENTATIONS IN FAB-MS MS SPECTRA OF GLYCOCONJUGATES
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DOI：
10.1007/bf01049915
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发表时间：
1988-01-01
影响因子：
3
通讯作者：
COSTELLO, CE
中科院分区：
文献类型：
--
作者：
DOMON, B;COSTELLO, CE
总结了糖缀合物（鞘糖脂、糖肽、糖苷和碳水化合物）的快原子轰击质谱（FAB-MS）和碰撞诱导分解（CID）MS/MS谱中观察到的离子类型。碳水化合物部分内裂解产生的产物离子类型的多样性促使我们引入一个系统的命名法来指定这些离子。所提出的命名法主要是为FAB-MS开发的，但也可用于其他电离技术[场解吸（FD）、直接化学电离（DCI）、激光解吸/傅里叶变换（LD/FT）等]，Ai、Bi和Cilabels用于表示含有末端（非还原性末端）糖单元的片段，而Xj、Yj和Zj表示仍含有糖苷配基（或还原性糖单元）的离子。下标表示相对于类似于肽中所用系统的末端的位置，上标表示碳水化合物环内的裂解。天然鞘糖脂和糖肽以及全甲基化神经节苷脂的FAB-MS/MS光谱如图所示。
A summary of the ion types observed in the Fast Atom Bombardment Mass Spectrometry (FAB-MS) and collision induced decomposition (CID) MS/MS spectra of glycoconjugates (glycosphingolipids, glycopeptides, glycosides and carbohydrates) is presented. The variety of product ion types that arise by cleavages within the carbohydrate moieties has prompted us to introduce a systematic nomenclature to designate these ions. The proposed nomenclature has been developed primarily for FAB-MS, but can be used as well for other ionization techniques [field desorption (FD), direct chemical ionization (DCI), laser desorption/Fourier transform (LD/FT), etc.], and is applicable to spectra recorded in either the positive or negative ion mode during both MS and MS/MS experiments.Ai, Biand Cilabels are used to designate fragments containing a terminal (nonreducing end) sugar unit, whereas Xj, Yjand Zjrepresent ions still containing the aglycone (or the reducing sugar unit). Subscripts indicate the position relative to the termini analogous to the system used in peptides, and superscripts indicate cleavages within carbohydrate rings.FAB-MS/MS spectra of a native glycosphingolipid and glycopeptide, and a permethylated ganglioside are shown as illustrations.