Mating pair combinations of insecticide-treated male and female Nilaparvata lugens Stal (Hemiptera: Delphacidae) planthoppers influence protein content in the male accessory glands (MAGs) and vitellin content in both fat bodies and ovaries of adult females
Mating pair combinations of insecticide-treated male and female Nilaparvata lugens Stal (Hemiptera: Delphacidae) planthoppers influence protein content in the male accessory glands (MAGs) and vitellin content in both fat bodies and ovaries of adult females
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DOI：
10.1016/j.pestbp.2010.06.019
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发表时间：
2010-10-01
影响因子：
4.7
通讯作者：
Huang, Liu-Juan
中科院分区：
文献类型：
--
作者：
Ge, Lin-Quan;Wang, Li-Ping;Huang, Liu-Juan
褐飞虱Nilparvata luens(Stal)(半翅目：飞蝇科)是一种成虫，其后代由成虫与成虫交配而成。这一物种是由杀虫剂引起害虫死灰复燃的典型案例。在过去，对复苏机制的研究主要集中在杀虫剂诱导的成年雌性生殖刺激上。然而，到目前为止，雄性在褐飞虱复苏机制中所起的作用还没有被研究过。本研究的目的是检测杀虫剂三唑磷和溴氰菊酯诱导的雄性副腺(MAG)蛋白质水平的变化，并在交配对的背景下确定它们与成年雌性脂肪体和卵巢中卵黄蛋白含量的关系。结果表明，雄虫交配状态、杀虫剂种类和杀虫剂浓度对MAGS的蛋白质含量有显著影响。杀虫剂的使用诱导MAG蛋白水平升高。大量的MAG产物通过交配转移到雌性体内。因此，交配后MAG中的蛋白质水平显著下降。实验结果表明，处理过的雄性与未处理过的雌性(雄性(T)×雌性(Ck))配对的成年雌性在脂肪体和卵巢中的卵黄素含量显著高于由未处理的雄性与未处理的雌性(雄性(Ck)×雌性(Ck))组成的交配对。在两种杀虫剂的不同浓度下，卵黄蛋白水平在由一只经过处理的雄性和一只经过处理的雌性(雄性(T)×雌性(T))组成的交配对中最高，其次是由一只未处理的雄性和一只经过处理的雌性(雄性(Ck)×雌性(T))组成的交配对。这些发现表明：(1)杀虫剂对雄性有影响；(2)杀虫剂的作用可以传递给雌性；(3)与只有一个个体得到处理的交配对相比，雄性和雌性都得到处理的交配对的生殖效果最强。这些发现提供了关于雄性在杀虫剂引起的褐飞虱死灰复燃中的作用的有价值的信息。(C)2010 Elsevier Inc.保留所有权利。
The brown planthopper, Nilaparvata lugens (Stal)(Hemiptera: Delphacidae), is an insect pest in which offspring are produced by the mating of adult males with adult females. This species is a classic case in which pest resurgence is induced by insecticides. In the past, studies of resurgence mechanisms have focused on insecticide-induced stimulation of reproduction in adult females. To date, however, the role that males play in the resurgence mechanisms of N. lugens has not been investigated. The aim of the present study is to examine changes in protein levels in male accessory glands (MAGs) induced by the insecticides triazophos and deltamethrin and to determine their relationship with vitellin content in the fat bodies and ovaries of adult females in the context of mating pairs. Our results show that protein content in MAGs is significantly affected by male mating status, insecticide type, and insecticide concentration. Insecticide application induced increased protein levels in MAGs. A greater quantity of MAG products was transferred to females via mating. Thus, protein levels in MAGs significantly decreased after mating. Experimental matings indicate that vitellin content in both fat bodies and ovaries of adult females in mating pairs consisting of a treated male and an untreated female (male(t), x female(ck)) is significantly greater than that of females in pairs consisting of an untreated male and an untreated female (male(ck) x female(ck)). Under various concentrations of the two insecticides, vitellin levels are highest in mating pairs consisting of a treated male and a treated female (male(t) x female(t)), followed by mating pairs consisting of an untreated male with a treated female (male(ck) x female(t)). These findings demonstrate that (1) insecticides have an effect on males; (2) insecticide effect can be transferred to females; and (3) the reproductive effect of insecticides is strongest in mating pairs in which both the males and females are treated compared to pairs in which only one individual is treated. These findings provide valuable information about the role of males in pesticide-induced resurgence of N. lugens. (C) 2010 Elsevier Inc. All rights reserved.