The influence of molecular weight, crosslinking and counterface roughness on TNF-alpha production by macrophages in response to ultra high molecular weight polyethylene particles
The influence of molecular weight, crosslinking and counterface roughness on TNF-alpha production by macrophages in response to ultra high molecular weight polyethylene particles
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DOI：
10.1016/j.biomaterials.2003.10.054
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Ingham, E
中科院分区：
文献类型：
--
作者：
Ingram, JH;Stone, M;Ingham, E
研究了小鼠巨噬细胞对不同聚乙烯在不同时间点和体积剂量下产生的临床相关聚乙烯磨损颗粒的反应。在无菌条件下，在RPMI 1640润滑剂中添加25% （v/v）胎牛血清，使用多向板钉磨损装置在体外产生具有临床意义的超高分子量聚乙烯（UHMWPE）磨损碎片。磨损碎片与C3H小鼠腹腔巨噬细胞以不同颗粒体积（mum(3)）：细胞数比培养。ELISA法检测tnf - α的分泌。首先考虑了超高分子量聚乙烯分子量的影响。高分子量GUR415HP比低分子量GUR1120具有更低的磨损率，但高分子量GUR415HP产生的磨损碎屑体积在0.1-1.01 μ m范围内。GUR415HP的磨损碎屑在浓度为1 μ m(3)/细胞时产生显著水平的TNF-a，而每个细胞至少需要10 μ m(3)/细胞的GUR1120磨损碎屑才能产生显著水平的TNF-a。其次，在磨损有划痕的台面时，研究了交联GUR1050的效果。材料的磨损率随交联水平的增加而降低。然而，与未交联的材料相比，经5和10 Mrad γ辐照交联的材料产生0.1-1.0 μ m尺寸磨损颗粒的百分比更高。虽然交联材料能够在颗粒浓度仅为0.1 μ m(3)/细胞时刺激细胞产生显著升高的tnf - α水平，但只有浓度为10 μ m(3)/细胞及以上的非交联磨损碎片具有刺激作用。当台面从划痕变为光滑时，所有三种GUR1050材料的磨损率都进一步降低。首次从聚乙烯中观察到纳米尺寸的磨损颗粒，这减少了0.1-1.0微米尺寸范围内碎片的百分比质量。对于光滑表面上的所有三种材料，只有50 mum(3)/细胞及以上的浓度具有刺激作用。本研究表明分子量、交联和表面粗糙度是决定聚乙烯生物活性的重要因素。(C) 2003 Elsevier Ltd.版权所有。
The response of murine macrophages to clinically relevant polyethylene wear particles generated from different polyethylenes at various time points and volumetric doses in vitro was evaluated. Clinically relevant ultra high molecular weight polyethylene (UHMWPE) wear debris was generated in vitro in a lubricant of RPMI 1640 supplemented with 25% (v/v) foetal calf serum using a multi-directional pin-on-plate wear rig under sterile conditions. Wear debris was cultured with C3H murine peritoneal macrophages at various particle volume (mum(3)): cell number ratios. The secretion of TNF-alpha was determined by ELISA.Initially the effect of molecular weight of UHMWPE was considered. Higher molecular weight GUR415HP was shown to have a lower wear rate than the lower molecular weight GUR1120, however a greater volume of the wear debris produced by the high molecular weight GUR415HP was in the 0.1-1.01mum size range. Wear debris from GUR415HP produced significant levels of TNF-a at a concentration of 1 mum(3)/cell while at least 10 mum(3)/cell of GUR1120 wear debris per cell was needed to produce significant levels of TNF-a. Secondly the effects of crosslinking GUR1050 was examined when worn against a scratched counterface. The wear rate of the material was shown to decrease as the level of crosslinking increased. However the materials crosslinked with 5 and 10 Mrad of gamma irradiation produced higher percentages of 0.1-1.0 mum size wear particles than the non-crosslinked material. While the crosslinked material was able to stimulate cells to produce significantly elevated TNF-alpha levels at a particle concentration of just 0.1 mum(3)/cell only concentrations of 10 mum(3)/cell and above of the non-crosslinked wear debris were stimulatory. When the counterface was changed from scratched to smooth the wear rate for all three GUR1050 materials was further reduced. For the first time nanometre size wear particles were observed from polyethylene which reduced the percentage mass of debris in the 0.1-1.0 mum size range. For all three materials on the smooth counterface only concentrations of 50 mum(3)/cell and above were stimulatory. This study has demonstrated that molecular weight, crosslinking and counterface roughness are important factors in determining the biological activity of polyethylene. (C) 2003 Elsevier Ltd. All rights reserved.