Nano Hydroxyapatite & Mineral Trioxide Aggregate Efficiently Promote Odontogenic Differentiation of Dental Pulp Stem Cells.
Nano Hydroxyapatite & Mineral Trioxide Aggregate Efficiently Promote Odontogenic Differentiation of Dental Pulp Stem Cells.
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DOI：
10.3889/oamjms.2018.368
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发表时间：
2018-09-25
影响因子：
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通讯作者：
Aly, Riham M
中科院分区：
文献类型：
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作者：
Hanafy, Ahmed Khaled;Shinaishin, Souzy F;Aly, Riham M
背景：传统的牙髓干细胞修复方法依赖人工合成材料来修复缺损区和缺失的组织，缺乏修复组织生理结构和功能的能力，因此迫切需要从传统的治疗方法转向更有前景的再生策略。目的：本研究旨在评价两种常用的生物材料，即三氧化二矿团聚体(MTA)和纳米羟基磷灰石作为牙髓干细胞(DPSCS)成牙分化的促进剂的作用。通过qRT-PCR示踪成牙本质细胞不同阶段的基因特征，评价成牙本质细胞的分化。结果：MTA和纳米羟基磷灰石均能促进DPSC的牙源性分化。结论：纳米羟基磷灰石具有较高的促进作用。然而，在没有牙源性介质的情况下，MTA和纳米羟基磷灰石不能促进DPSCs的成牙分化。
BACKGROUND: There has been an urge to shift from conventional therapies to the more promising regenerative strategy since conventional treatment relies on synthetic materials to fill defects and replace missing tissues, lacking the ability to restore the tissues' physiological architecture and function.AIM: The present study focused on the assessment of the role of two commonly used biomaterials namely; mineral trioxide aggregate (MTA) and nano hydroxy-apatite as promoters of odontogenic differentiation of dental pulp stem cells (DPSCs).METHODS: DPSCs were isolated, cultured in odontogenic media and divided into three groups; control group, MTA group and nanohydroxyapatite group. Odontogenic differentiation was assessed by tracing genes characteristic of different stages of odontoblasts via qRT-PCR. Calcific nodules formation was evaluated by Alizarin red staining.RESULTS: Results demonstrated that both MTA and nanohydroxyapatite were capable of enhancing odontogenic differentiation of DPSCs.CONCLUSION: Nano hydroxyapatite was found to have a higher promoting effect. However, in the absence of an odontogenic medium, MTA and nanohydroxyapatite could not enhance the odontogenic differentiation of DPSCs.