Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues
Low antioxidant enzyme gene expression in pancreatic islets compared with various other mouse tissues
复制标题
DOI：
10.1016/0891-5849(96)02051-5
复制
发表时间：
1996-01-01
影响因子：
7.4
通讯作者：
Tiedge, M
中科院分区：
文献类型：
--
作者：
Lenzen, S;Drinkgern, J;Tiedge, M
用一种灵敏的Northern杂交技术，研究了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPO)基因在胰岛和其他不同组织(肝、肾、脑、肺、骨骼肌、心肌、肾上腺和脑下垂体)中的表达。抗氧化酶的基因表达通常在肝脏的+/-50%的范围内。只有在胰岛中，基因表达水平显著降低。细胞质铜/锌超氧化物歧化酶和线粒体锰超氧化物歧化酶基因表达水平约为肝脏的30-40%。胰岛谷胱甘肽过氧化物酶基因表达为15%，过氧化氢酶基因表达为阴性。这些抗氧化酶基因的低水平表达可能解释了为什么胰腺β细胞对糖尿病原化合物的细胞毒性损伤以及在人类和动物糖尿病的发展过程中具有特殊的敏感性。
Using a sensitive Northern blot hybridization technique, gene expression of superoxide dismutase (SOD), catalase, and glutathione peroxidase was studied in pancreatic islets and for comparison in various other mouse tissues (liver, kidney, brain, lung, skeletal muscle, heart muscle, adrenal gland, and pituitary gland). Gene expression of the antioxidant enzymes was usually in the range of +/-50% of that in the liver. Only in pancreatic islets gene expression was substantially lower. The levels of the cytoplasmic Cu/Zn SOD and the mitochondrial Mn SOD gene expression were in the range of 30-40% of those in the liver. Glutathione peroxidase gene expression was 15%, and catalase gene expression was not at all detectable in pancreatic islets. These low levels of antioxidant enzyme gene expression may provide an explanation for the extraordinary sensitivity of pancreatic beta cells towards cytotoxic damage by diabetogenic compounds and during the development of human and animal diabetes.