Advanced modular automated calculation of the morpho-histological parameters in myocardial infarction.
Advanced modular automated calculation of the morpho-histological parameters in myocardial infarction.
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DOI：
10.15190/d.2016.13
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发表时间：
2016-09-30
期刊：
影响因子：
--
通讯作者：
Liehn, Elisa A
中科院分区：
文献类型：
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作者：
Curaj, Adelina;Pop-Fele, Lucian;Liehn, Elisa A
心肌梗死是被研究最多的病理。心脏组织在临床实验室的常规基础上进行组织学检测。然而，缺乏用于例如计算心肌梗死面积大小的标准手术程序可能导致误差间隔增加，在相同组织的结果和/或与其他病理生理参数之间几乎没有相关性。这为进一步开发新的治疗策略创造了明显的障碍。在本研究中，我们展示了一种新方法的强大适用性，例如：先进的模块化自动计算(I)小鼠梗死心脏的大小，(Ii)瘢痕中存在的净胶原含量，以及(Iii)远程的间质纤维化，这两种方法都是同时进行的。作为每一次心脏治疗干预的重要预测指标之一，定义梗塞面积的新方法将对研究的准确性产生积极的影响。此外，预计容易汇编的同时计算的参数的报告及其用户友好的操作允许对测量进行有效和有效的估计。
Myocardial infarction represents the most investigated pathology. Heart tissues are histologically assayed on a routine base in clinical laboratories. However, the lack of a standard operation procedure for e.g. calculating the size of the infarcted area of myocardium, may lead to an increased errors' interval, with little correlation between results of a same tissue and/or with other pathophysiological parameters. This creates a clear barrier for further development of novel therapeutic strategies. In the present study, we present the robust applicability of a novel methodology such as: advanced modular automated calculation of (i) the size of infarcted heart of mice, (ii) the net collagen content present in the scar, and (iii) the interstitial fibrosis in remote, which are simultaneously performed. The new approach of defining the infarct size, one of the important predictor of every cardiac therapeutic intervention, will create a positive impact in the research accuracy. Additionally, it will be expected that the readily assembled reports of simultaneously computed parameters and its user-friendly operation allow an efficient and effective estimation of measurements.