Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling.
Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling.
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体内纳米金刚石的光学检测磁共振；
DOI：
10.1166/jnn.2015.9739
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
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作者：
Yoshinari;Y.;Mori;S.;Igarashi;R.;Sugi;T.;Yokota;H.;Ikeda;K.;Sumiya;H.;Mori;I;Tochio;H.;*Harada;Y. and Shirakawa;M.
生物样品的单分子荧光测量经常受到来自活体样品中存在的荧光材料的本底自身荧光和荧光标记分子不稳定的光物理性质的影响。在这项研究中，我们首先描述了我们的方法选择性成像纳米金刚石含有氮空位中心，有前途的荧光色中心，结合光学检测磁共振。所得到的图像在实时显微镜观察中完美地消除了外来的荧光。作为一个应用于In Vivo系统的实际例子，我们测量了在清晰背景下引入秀丽隐杆线虫肠道的纳米金刚石的共振光谱，并比较了随时间的光谱曲线。观察到的演变强烈表明，纳米金刚石的旋转被检测到。我们还报告了我们最近在研制一种配备雪崩光电二极管的光谱仪方面取得的进展，该光谱仪可用于光子的快速采样，可用于实时观察视场的选择性图像。该仪器适用于通过测量纳米金刚石旋转引起的荧光强度波动来探索动力学。
Single-molecule fluorescence measurements of biological samples frequently suffer from background autofluorescence originating from fluorescent materials pre-existing in living samples, and from unstable photo-physical properties of fluorescent labeling molecules. In this study, we first describe our method of selective imaging of nanodiamonds containing nitrogen-vacancy centers, promising fluorescent color centers, by a combination of optically detected magnetic resonance. The resultant images exhibit perfect elimination of extraneous fluorescence in real-time microscope observations. As the practical example applied to an In Vivo system, we measured the resonance spectrum of nanodiamonds introduced into the intestine of Caenorhabditis elegans in the clear background and compared the spectral profile over time. The observed evolution strongly suggests that the rotation of the nanodiamond was detected. We also report our recent progress in the development of a spectrometer equipped with an avalanche photo-diode for fast sampling of photons, which can be used while observing the selective image of a field of view in a real-time manner. This apparatus is suitable for exploring dynamics through the measurement of fluctuation in fluorescence intensity caused by a rotating nanodiamond.