Osmotic stress increases brain-derived neurotrophic factor messenger RNA expression in the hypothalamic supraoptic nucleus with differential regulation of its transcripts. Relation to arginine-vasopressin content
Osmotic stress increases brain-derived neurotrophic factor messenger RNA expression in the hypothalamic supraoptic nucleus with differential regulation of its transcripts. Relation to arginine-vasopressin content
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DOI：
10.1016/s0306-4522(02)00128-8
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Tapia-Arancibia, L
中科院分区：
文献类型：
--
作者：
Aliaga, E;Arancibia, S;Tapia-Arancibia, L
我们已经表明，渗透压增加视上核的脑源性神经营养因子（BDNF）mRNA，这种增加似乎是由含有外显子I的转录本的高表达决定的。室旁核是另一个下丘脑神经元亚群，BDNF mRNA对渗透压刺激也很敏感，在这个核中，含有外显子I的转录本没有被修饰，而只有含有外显子IT的转录本被修饰。这些核团对渗透刺激非常敏感，它们的神经元在垂体后叶分泌催产素和垂体后叶加压素。本研究旨在探讨BDNF是否参与视上核对渗透压应激的反应。在分析前3 h，通过向动物注射高渗盐水（1.35%NaCl）诱导渗透应激。我们使用非同位素原位杂交研究BDNF mRNA的表达及其转录与反义核糖核酸探针的组织学脑切片，包括室旁核和视上核从控制和渗透应激刺激的动物。为探讨BDNF mRNA表达与垂体后叶素之间可能存在的相关性，采用免疫组织化学方法分析了高渗注射后两个不同时间点室旁核和视上核中BDNF mRNA和垂体后叶素的含量。结果表明，BDNF mRNA的表达先于精氨酸加压素的增加。此外，在一系列相邻的视上核（10妈妈）的组织切片，BDNF和精氨酸加压素mRNA的同位素原位杂交和重叠的图像，显示几乎所有的杂交信号是overlapped.Together我们的结果是符合假设，激活不同的BDNF启动子似乎是区域特异性的。此外，BDNF mRNA的表达和垂体后叶素含量之间的时间相关性，以及它们各自的生产细胞之间的形态学附近的视上核，建议自分泌或旁分泌的行动，这种神经营养调节垂体后叶素分泌。(C)2002年IBRO。由Elsevier Science Ltd.出版，版权所有。
We have shown that osmotic stress increases brain-derived neurotrophic factor (BDNF) mRNA in the supraoptic nucleus and that this increase seems to be determined by the high expression of transcripts containing exon I. The paraventricular nucleus is another hypothalamic neuronal subset where BDNF mRNA is also sensitive to osmotic stress stimulation, In this nucleus, transcripts containing exon I were not modified but only those containing exon IT. By contrast, transcripts containing exon III did not exhibit any variation in our experimental conditions.The presence of BDNF mRNA in both paraventricular and supraoptic hypothalamic nuclei was recently reported. These nuclei are extremely sensitive to osmotic stimuli and their neurons secrete oxytocin and arginine-vasopressin in the posterior pituitary gland. This study was thus designed to investigate the possible involvement of BDNF in the response of supraoptic nucleus to osmotic stress stimulus. Osmotic stress was induced by hypertonic saline injection (1.35% NaCl) administered to animals 3 h before analysis. We used non-isotopic in situ hybridization to study the expression of BDNF mRNA and its transcripts with antisense riboprobes on histological brain sections, including paraventricular and supraoptic nuclei from control and osmotic stress-stimulated animals. To investigate a possible correlation between the expression of BDNF mRNA and arginine-vasopressin, the peptide content was analyzed by immunohistochemistry in both paraventricular and supraoptic nuclei at two different times after hyperosmotic injection. The results showed that BDNF mRNA expression preceded the arginine vasopressin increase. In addition, on serial adjacent histological sections of supraoptic nucleus (10 mum), both BDNF and arginine-vasopressin mRNAs were visualized by isotopic in situ hybridization and the images were overlaid, showing that almost all of the hybridization signals were overlapped.Taken together our results are in keeping with the hypothesis that activation of the different BDNF promoters seems to be region-specific. Besides, the temporal correlation between both BDNF mRNA expression and arginine-vasopressin content, as well as the morphological vicinity between their respective producing cells in the supraoptic nucleus, suggest an autocrine or paracrine action for this neurotrophin in the regulation of arginine-vasopressin secretion. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.