Performance and Limitations of Phosphate Quantification: Guidelines for Plant Biologists
Performance and Limitations of Phosphate Quantification: Guidelines for Plant Biologists
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DOI：
10.1093/pcp/pcv208
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发表时间：
2016-04-01
影响因子：
4.9
通讯作者：
Nussaume, Laurent
中科院分区：
文献类型：
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作者：
Kanno, Satomi;Cuyas, Laura;Nussaume, Laurent
磷酸盐（Pi）是植物生命所必需的大量营养素。已经确定了几个参与Pi稳态的调节成分，揭示了细胞和亚细胞水平上非常高的复杂性。确定植物中π的含量对于理解这一调控至关重要，短时间实时(33)π吸收成像实验表明π的运动是高度动态的。此外，Pi的基因调节是由该离子在组织以及细胞和亚细胞水平的定位精细控制的。破译这些规则需要访问和量化Pi池在各个植物隔间。本文综述了测量、可视化和追踪植物中Pi的不同技术，并对未来的前景进行了讨论。
Phosphate (Pi) is a macronutrient that is essential for plant life. Several regulatory components involved in Pi homeostasis have been identified, revealing a very high complexity at the cellular and subcellular levels. Determining the Pi content in plants is crucial to understanding this regulation, and short real-time (33)Pi uptake imaging experiments have shown Pi movement to be highly dynamic. Furthermore, gene modulation by Pi is finely controlled by localization of this ion at the tissue as well as the cellular and subcellular levels. Deciphering these regulations requires access to and quantification of the Pi pool in the various plant compartments. This review presents the different techniques available to measure, visualize and trace Pi in plants, with a discussion of the future prospects.