A novel gene expressed during zebrafish gastrulation identified by differential RNA display.
A novel gene expressed during zebrafish gastrulation identified by differential RNA display.
复制标题
通过差异 RNA 显示鉴定斑马鱼原肠胚形成过程中表达的新基因。
DOI：
10.1016/0925-4773(95)00416-x
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发表时间：
1995
影响因子：
2.6
通讯作者：
Conway,G
中科院分区：
文献类型：
--
作者：
Conway,G
脊椎动物原肠胚形成是一个动态的发展时期，其特征是广泛的细胞迁移。这一发展阶段可能需要表达一种新的基因库，以启动和指导这些戏剧性的变化。差异RNA展示已被用于鉴定在模式脊椎动物斑马鱼原肠胚阶段特异表达的基因。通过差异显示技术分离的基因之一已被测序并表征其空间和时间表达。这种基因被称为G12，在原肠胚形成期间的一个狭窄的时间窗口内表达，并且仅限于单一细胞类型。在这个发育阶段，斑马鱼胚胎由三种细胞类型组成：卵黄细胞，EVL细胞和深层细胞。有趣的是，EVL和深层细胞在发育早期都来自共同的祖细胞，但G12表达仅限于EVL谱系。该基因的氨基酸序列与Genbank数据库的比较表明与两个先前报道的哺乳动物基因相似。这三个基因之间的相似性表明它们可能具有共同的功能。G12基因是斑马鱼EVL细胞中限制性基因表达的第一个例子。G12基因应被证明是一个有用的模型，在原肠胚形成过程中的基因表达调控的研究。
Vertebrate gastrulation is a dynamic period of development characterized by extensive cell migrations. This stage of development is likely to require the expression of a new genetic repertoire to initiate and direct these dramatic changes. The differential RNA display has been used to identify genes specifically expressed during the gastrula stage of a model vertebrate, the zebrafish. One of the genes isolated by the differential display technique has been sequenced and characterized for its spatial and temporal expression. This gene, called G12, is expressed during a narrow window of time during gastrulation and is restricted to a single cell type. At this time of development the zebrafish embryo consists of three cell types: the yolk cell, EVL cells and deep cells. Interestingly, both EVL and deep cells derive early in development from common progenitor cells but G12 expression is restricted only to the EVL lineage. Comparison of the amino acid sequence from this gene with the Genbank database indicates similarity to two previously reported mammalian genes. The similarity between these three genes suggests that they may serve a common function. The G12 gene is the first example of restricted gene expression in EVL cells of the zebrafish. The G12 gene should prove to be a useful model for the study of regulated gene expression during gastrulation.