Neuron-specific Hu proteins sub-cellular localization in primary sensory neurons
Neuron-specific Hu proteins sub-cellular localization in primary sensory neurons
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DOI：
10.1016/j.aanat.2006.11.004
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Giacobini-Robecchi, Maria Giuseppina
中科院分区：
文献类型：
--
作者：
Fornaro, Michele;Raimondo, Stefania;Giacobini-Robecchi, Maria Giuseppina
Hu家族rna结合蛋白参与了许多神经系统发育和维持的转录后机制。Hu家族的三个成员（HuB， HuC和HuD）是神经元特异性蛋白。在这项研究中，我们利用光镜和电镜显示了大鼠背根神经节（DRG）初级感觉神经元中神经元特异性Hu蛋白的亚细胞定位。利用这些技术，我们从形态学上揭示了细胞核和细胞质中神经元特异性Hu蛋白的存在，并区分了不同细胞器（特别是线粒体和高尔基体）中Hu蛋白的存在，从而支持了之前的观点，即NS-Hu蛋白使RNA与亚细胞器相互作用，并可能参与mRNA的细胞定位。(c) 2006 Elsevier GmbH版权所有。
The Hu family of RNA-binding proteins is involved in many post-transcriptional mechanisms for the development and maintenance of the nervous system. Three members of the Hu family (HuB, HuC and HuD) are neuron-specific proteins. In this study, we present data using light and electron microscopy to show the sub-cellular localization of neuron-specific Hu proteins in rat primary sensory neurons taken from dorsal root ganglia (DRG). Using these techniques we morphologically revealed the presence of neuron-specific-Hu proteins in the nucleus and in the cytoplasm and discriminated the presence of Hu proteins within different cellular organelles, specifically mitochondria and Golgi apparatus, thus supporting previous ideas that NS-Hu proteins enable RNA interactions with sub-cellular organelles and may be involved in mRNA cellular localization. (c) 2006 Elsevier GmbH. All rights reserved.