Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays
Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays
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DOI：
10.1038/sj.onc.1206247
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发表时间：
2003-02-27
期刊：
影响因子：
8
通讯作者：
Cooper, CS
中科院分区：
文献类型：
--
作者：
Clark, J;Edwards, S;Cooper, CS
我们证明，比较基因组杂交（CGH）到cDNA微阵列可用于进行全基因组筛选的遗传丢失的区域，包括纯合（完全）缺失，可能代表肿瘤抑制基因在人类癌症的可能位置。前列腺癌细胞系LNCaP、PC 3和DU 145的筛选允许对基因组拷贝数出现改变的特定区域进行定位，并导致在PC 3细胞系中鉴定出在17q21.31（跨越STAT 3的500 kb）和在10q23.1（跨越SFTPA 2的50-350 kb）处完全丢失的两个新区域。
We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21.31 (500 kb spanning STAT3) and at 10q23.1 (50-350 kb spanning SFTPA2) in the PC3 cell line.