On-demand droplet spotter for preparing pico- to femtoliter droplets on surfaces
On-demand droplet spotter for preparing pico- to femtoliter droplets on surfaces
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DOI:
10.1021/ac0012039
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发表时间:
2001-04-15
影响因子:
7.4
通讯作者:
Ishikawa, M
中科院分区:
文献类型:
--
作者:
Yogi, O;Kawakami, T;Ishikawa, M
A droplet spotter for on-demand generation of pico- to femtoliter droplets was developed to meet the requirement for high-density spotting of chemicals on a surface, Our droplet spotter involves applying a similar to 1000-V and similar to 10ms pulse voltage to the tip of a capillary tube (o.d. similar to 18 mum; i.d. similar to 11 pm) supplied with water or a dye solution. The capability of the spotter was demonstrated by preparing a microarray of dye molecules. The microarray was prepared by spotting similar to 30-fL droplets of a dye solution on a surface at the density of one spot per 20 x 20 mum(2).