On-demand droplet spotter for preparing pico- to femtoliter droplets on surfaces

On-demand droplet spotter for preparing pico- to femtoliter droplets on surfaces
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DOI:
10.1021/ac0012039
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发表时间:
2001-04-15
影响因子:
7.4
通讯作者:
Ishikawa, M
Ishikawa, M
中科院分区:
化学1区
文献类型:
--
作者:
Yogi, O;Kawakami, T;Ishikawa, M

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一种用于按需生成微升到飞升液滴的液滴检测仪被开发出来,以满足表面上高密度化学物质的检测要求。我们的液滴检测仪包括将类似于1000 v和类似于10ms的脉冲电压施加到毛细管的尖端(od值类似于18 μ m; id值类似于11 μ m),并提供水或染料溶液。通过制备染料分子微阵列,证明了该观察器的能力。微阵列的制备方法是将类似于30-fL的染料溶液滴在表面上,密度为每20 x 20 mum一个点(2)。
A droplet spotter for on-demand generation of pico- to femtoliter droplets was developed to meet the requirement for high-density spotting of chemicals on a surface, Our droplet spotter involves applying a similar to 1000-V and similar to 10ms pulse voltage to the tip of a capillary tube (o.d. similar to 18 mum; i.d. similar to 11 pm) supplied with water or a dye solution. The capability of the spotter was demonstrated by preparing a microarray of dye molecules. The microarray was prepared by spotting similar to 30-fL droplets of a dye solution on a surface at the density of one spot per 20 x 20 mum(2).