Sensitive CSF ELISAs for the detection of MBL, MASP-2 and functional MBL/MASP-2.
Sensitive CSF ELISAs for the detection of MBL, MASP-2 and functional MBL/MASP-2.
复制标题
用于检测 MBL、MASP-2 和功能性 MBL/MASP-2 的灵敏 CSF ELISA。
DOI：
10.1016/j.jneumeth.2012.06.004
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发表时间：
2012
影响因子：
3
通讯作者：
Singh,KumudK
中科院分区：
文献类型：
--
作者：
Kwok,JanetY;Augst,RyanM;Yu,DeniseY;Singh,KumudK
Mannose binding lectin (MBL) mediated complement pathway is an important constituent of innate immune response in several infections including neuroinflammatory and neurodegenerative diseases. Although there are Enzyme-Linked Immunosorbent Assays (ELISAs) for estimating MBL, MBL-associated serine protease-2 (MASP-2) and functional MBL-MASP-2 (fMBL) proteins for the plasma, serum and cell supernatants there are no established methods for their estimation in the cerebrospinal fluid (CSF).我们开发了灵敏的 ELISA 来检测 CSF 中的 MBL、fMBL 和 MASP-2。首先，我们采用标准 ELISA 来检测脑脊液中的这些蛋白质。 Second, we used a biotinyl-tyramide based horseradish peroxidase (HRP) signal amplification for the sensitive detection of these proteins in the CSF. In summary, using modified ELISA and biotinyl-tyramide based HRP signal amplification, we successfully detected MBL, fMBL and MASP-2 proteins in the CSF samples with high sensitivity and reproducibility.
Mannose binding lectin (MBL) mediated complement pathway is an important constituent of innate immune response in several infections including neuroinflammatory and neurodegenerative diseases. Although there are Enzyme-Linked Immunosorbent Assays (ELISAs) for estimating MBL, MBL-associated serine protease-2 (MASP-2) and functional MBL-MASP-2 (fMBL) proteins for the plasma, serum and cell supernatants there are no established methods for their estimation in the cerebrospinal fluid (CSF). We developed sensitive ELISAs for the detection of MBL, fMBL and MASP-2 in the CSF. First, we adapted standard ELISAs for the detection of these proteins in the CSF. Second, we used a biotinyl-tyramide based horseradish peroxidase (HRP) signal amplification for the sensitive detection of these proteins in the CSF. In summary, using modified ELISA and biotinyl-tyramide based HRP signal amplification, we successfully detected MBL, fMBL and MASP-2 proteins in the CSF samples with high sensitivity and reproducibility.