Effects of oxygen radicals on cerebral arterioles.
Effects of oxygen radicals on cerebral arterioles.
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DOI：
10.1152/ajpheart.1985.248.2.h157
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发表时间：
1985-02
期刊：
影响因子：
--
通讯作者：
E. P. Wei;C. W. Christman;H. Kontos;J. Povlishock
中科院分区：
文献类型：
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作者：
E. P. Wei;C. W. Christman;H. Kontos;J. Povlishock
黄嘌呤氧化酶和黄嘌呤（一种产生过氧化氢和超氧阴离子自由基的组合）局部应用于配备有颅窗的麻醉猫，在应用过程中引起小动脉扩张，洗脱后1 h持续扩张，对动脉低碳酸血症的血管收缩作用的反应性降低，内皮离散病变和电子显微镜下血管平滑肌形态异常。在局部应用过氧化氢或过氧化氢加硫酸亚铁（一种产生自由羟基的组合）期间，在小动脉中观察到类似的效果，但在大动脉中没有观察到。超氧化物歧化酶和过氧化氢酶可完全抑制黄嘌呤氧化酶和黄嘌呤引起的功能变化。超氧化物歧化酶或过氧化氢酶本身消除了洗脱后观察到的残留效应，并减少了应用黄嘌呤氧化酶期间的扩张。结果表明，超氧阴离子自由基和过氧化氢产生可逆的小动脉扩张，并在超氧阴离子自由基和过氧化氢的存在下产生一致的血管损伤。
Xanthine oxidase and xanthine, a combination that produces hydrogen peroxide and superoxide anion radical, applied topically in anesthetized cats equipped with cranial windows caused arteriolar dilation during application, sustained dilation 1 h after washout, and reduced reactivity to the vasoconstrictive effects of arterial hypocapnia, discrete lesions of the endothelium, and morphological abnormalities of the vascular smooth muscle by electron microscopy. Similar effects were seen in small, but not in large, arterioles during topical application of hydrogen peroxide or hydrogen peroxide plus ferrous sulfate, a combination that produces free hydroxyl radical. The functional changes caused by xanthine oxidase plus xanthine were inhibited completely by superoxide dismutase plus catalase. Superoxide dismutase or catalase, each by itself, eliminated the residual effects seen after washout and reduced the dilation during application of xanthine oxidase. The results show that superoxide anion radical and hydrogen peroxide produce reversible arteriolar dilation and that consistent vascular damage is produced in the presence of both superoxide anion radical and hydrogen peroxide.