Whole blood platelet aggregation in humans and animals: A comparative study
Whole blood platelet aggregation in humans and animals: A comparative study
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DOI：
10.1006/jsre.1998.5543
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发表时间：
1999-04-01
影响因子：
2.2
通讯作者：
Harasaki, H
中科院分区：
文献类型：
--
作者：
Soloviev, MV;Okazaki, Y;Harasaki, H
背景许多动物种属被用于评价心血管器械的性能和血液相容性，但血小板活性的种属间差异尚未得到很好的表征。本研究测量人、狗和小牛血液中血小板对六种激动剂的反应。我们使用全血阻抗发光聚集测定法测量成年人（n = 19）、杂种犬（n = 19）和荷斯坦小牛（n = 7）血样中的血小板聚集和ATP释放。激动剂为胶原蛋白、维生素C、花生四烯酸、凝血酶以及三种浓度的ADP和肾上腺素。只有胶原蛋白（1 μ g/ml）和ADP（5，10和20 μ M）引起的聚集和ATP释放在所有样品。犬血小板对所有剂量的所有6种激动剂均有反应。人血小板对除2 μ M和100 μ M肾上腺素外的所有物质都有反应.牛血小板仅对胶原蛋白、ADP和凝血酶有反应。在牛血小板中，胶原蛋白的聚集和凝血酶的ATP释放显着低于人和犬血液中的相应反应。10 μ M ADP诱导的犬血小板聚集率显著高于人血小板。结论人、犬和牛血小板对激动剂的反应有很大差异。在这些模型中，胶原蛋白（1 μ g/ml）和ADP（10 μ M）是研究全血血小板聚集的首选激动剂，因为它们提供了物种之间最一致的结果。对于ATP释放，1 U/ml凝血酶是推荐的激动剂和所有三种物种的剂量，（C）1999 Academic Press。
Background. Many animal species are used to evaluate the performance and blood compatibility of cardiovascular devices, but interspecies differences in platelet activity have not been well characterized. This study measures platelet response to six agonists in human, dog, and calf blood.Materials and methods. We used whole blood impedance lumi-aggregometry to measure platelet aggregation and ATP release in blood samples from adult humans (n = 19), mongrel dogs (n = 19), and Holstein calves (n = 7). The agonists were collagen, ristocetin, arachidonic acid, thrombin, and three concentrations of both ADP and epinephrine.Results. Only collagen (1 mu g/ml) and ADP (5, 10, and 20 mu M) caused aggregation and ATP release in all samples. Canine platelets responded to all six agonists at all doses. Human platelets responded to everything except epinephrine at 2 and 100 mu M. Bovine platelets responded only to collagen, ADP, and thrombin. In bovine platelets, aggregation from collagen and ATP release from thrombin were significantly lower than the corresponding responses in human and canine blood. The aggregation induced by 10 mu M ADP was significantly higher in canine than in human platelets.Conclusion, Human, canine, and bovine platelets have very different responses to agonists. In these models, collagen (1 mu g/ml) and ADP (10 mu M) are the agonists of choice for investigating whole blood platelet aggregation because they provide the most consistent results between species. For ATP release, 1 U/ml thrombin is the recommended agonist and the dose for all three species, (C) 1999 Academic Press.