Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay.
Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay.
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激活素对雌性大鼠垂体细胞激素分泌的影响：细胞免疫印迹分析。
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
Toshihiro Aono
中科院分区：
文献类型：
--
作者：
S. Miyamoto;M. Irahara;K. Ushigoe;A. Kuwahara;Hiromu Sugino;Toshihiro Aono
我们研究了激活素A对分泌的LH，FSH和催乳素（PRL）的雌性大鼠垂体细胞在单细胞水平上通过细胞免疫印迹分析。将来自8周龄雌性大鼠的垂体前叶细胞与或不与激活素A预孵育24小时，之后将其单分散并立即用于细胞免疫印迹测定。LH-，FSH-和PRL-免疫反应细胞印迹检测的百分比分别为5.5%，5.3%和43.1%，所有垂体细胞适用于转移膜。LH分泌细胞的百分比和每个细胞的平均LH分泌量在用激活素处理后没有变化。相反，激活素显著增加FSH分泌细胞的百分比和每个细胞的平均FSH分泌量至136.0和114。5%。此外，激活素显著降低PRL分泌细胞的百分比和每个细胞的平均PRL分泌量，分别为52.2%和72.0%。这些结果表明：（1）激活素A对雌性大鼠垂体细胞具有作用，不仅增加每个细胞的FSH分泌量，而且增加FSH分泌细胞的数量;（2）激活素A降低每个细胞的PRL分泌量和PRL分泌细胞的数量。
We investigated the effect of activin A on secretion of LH, FSH, and prolactin (PRL) by female cultured rat pituitary cells at the single-cell level by means of the cell immunoblot assay. Anterior pituitary cells from 8-week-old female rats were preincubated with or without activin A for 24 h, after which they were monodispersed and immediately used for cell immunoblot assay. The percentages of LH-, FSH- and PRL-immunoreactive cell blots detected were 5.5, 5.3 and 43.1%, respectively, of all pituitary cells applied to the transfer membrane. The percentage of LH-secreting cells and mean LH secretion per cell did not change after treatment with activin. In contrast, activin significantly increased the percentage of FSH-secreting cells and mean FSH secretion per cell to 136.0 and 114. 5% respectively. In addition, activin significantly decreased the percentage of PRL-secreting cells and mean PRL secretion per cell to 52.2 and 72.0% respectively. These results suggest that (1) activin A has effects on female rat pituitary cells that increase not only the amount of FSH secretion per cell but also the number of FSH-secreting cells, and (2) activin A decreases both the amount of PRL secretion per cell and the number of PRL-secreting cells.
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DOI：
10.1210/endo.137.9.8756531
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期刊：
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DOI：
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发表时间：
1991
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
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作者：
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影响因子：
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作者：
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