Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway.
Assembly of functional photosystem complexes in Rhodobacter sphaeroides incorporating carotenoids from the spirilloxanthin pathway.
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DOI：
10.1016/j.bbabio.2014.10.004
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发表时间：
2015-02
影响因子：
4.3
通讯作者：
Hunter, C. Neil
中科院分区：
文献类型：
--
作者：
Chi, Shuang C.;Mothersole, David J.;Dilbeck, Preston;Niedzwiedzki, Dariusz M.;Zhang, Hao;Qian, Pu;Vasilev, Cvetelin;Grayson, Katie J.;Jackson, Philip J.;Martin, Elizabeth C.;Li, Ying;Holten, Dewey;Hunter, C. Neil
类胡萝卜素保护光合机构免受光和氧存在下产生的有害自由基的侵害。它们还作为辅助色素用于收集太阳能，并且是许多捕光复合物稳定组装所需的。光合细菌Rhodobacter（Rba.）类球胡萝卜素八氢番茄红素去饱和酶（CrtI）催化无色类胡萝卜素八氢番茄红素的三个连续的去饱和，将共轭碳-碳双键的数目N从3扩展到9，并产生黄色类胡萝卜素脉孢烯;随后的修饰产生黄色/红色类胡萝卜素类球烯/类球番茄红素酮（N = 10/11）。使用基因组crtI替换来交换天然三步Rba。sphaeroides CrtI的四步成团泛菌酶，其重新路由类胡萝卜素的生物合成，并最终在2，2 ′-二酮基-三尖杉黄素的生产在半有氧条件下。新的类胡萝卜素途径，阐明了使用HPLC和质谱的组合。通过灭活crtC或crtD产生以番茄红素或视紫红质为主要类胡萝卜素的菌株而过早终止这种新途径。LH 2和RC-LH 1-PufX复合物的组装途径接受所有的类胡萝卜素。LH 2复合物中2，2 ′-二酮基黄质（15个共轭CC键; N = 15）的类胡萝卜素-细菌叶绿素能量转移效率较低，为35%。对于脉孢烯（N = 9; 94%）、类球孢烯（N = 10; 96%）和球孢酮（N = 11; 95%）获得高能量转移效率，而对于番茄红素（N = 11; 64%）、视紫红质（N = 11; 62%）和大豆黄素（N = 13; 39%）测量中间值。这些新的Rba的多样性和稳定性。球形天线复合体使它们成为研究细菌光合作用中类胡萝卜素能量传递动力学的有用实验模型。在Rba中构建了油菜黄素生物合成途径。sphaeroides新的类胡萝卜素被光系统组装途径所接受。这些颜料被有效地整合到LH 2和RC-LH 1-PufX复合物中。类胡萝卜素-BChl能量转移随着共轭CC键的数目（N）而下降。N = 15的类胡萝卜素2，2 ′-二酮基黄质的效率最低，为35%。
Carotenoids protect the photosynthetic apparatus against harmful radicals arising from the presence of both light and oxygen. They also act as accessory pigments for harvesting solar energy, and are required for stable assembly of many light-harvesting complexes. In the phototrophic bacterium Rhodobacter (Rba.) sphaeroides phytoene desaturase (CrtI) catalyses three sequential desaturations of the colourless carotenoid phytoene, extending the number of conjugated carbon–carbon double bonds, N, from three to nine and producing the yellow carotenoid neurosporene; subsequent modifications produce the yellow/red carotenoids spheroidene/spheroidenone (N = 10/11). Genomic crtI replacements were used to swap the native three-step Rba. sphaeroides CrtI for the four-step Pantoea agglomerans enzyme, which re-routed carotenoid biosynthesis and culminated in the production of 2,2′-diketo-spirilloxanthin under semi-aerobic conditions. The new carotenoid pathway was elucidated using a combination of HPLC and mass spectrometry. Premature termination of this new pathway by inactivating crtC or crtD produced strains with lycopene or rhodopin as major carotenoids. All of the spirilloxanthin series carotenoids are accepted by the assembly pathways for LH2 and RC–LH1–PufX complexes. The efficiency of carotenoid-to-bacteriochlorophyll energy transfer for 2,2′-diketo-spirilloxanthin (15 conjugated CC bonds; N = 15) in LH2 complexes is low, at 35%. High energy transfer efficiencies were obtained for neurosporene (N = 9; 94%), spheroidene (N = 10; 96%) and spheroidenone (N = 11; 95%), whereas intermediate values were measured for lycopene (N = 11; 64%), rhodopin (N = 11; 62%) and spirilloxanthin (N = 13; 39%). The variety and stability of these novel Rba. sphaeroides antenna complexes make them useful experimental models for investigating the energy transfer dynamics of carotenoids in bacterial photosynthesis. The spirilloxanthin biosynthetic pathway has been constructed in Rba. sphaeroides. The new carotenoids are accepted by the photosystem assembly pathways. These pigments are efficiently integrated into LH2 and RC–LH1–PufX complexes. Carotenoid–BChl energy transfer drops with the number of conjugated CC bonds (N). The lowest efficiency, 35%, is for the N = 15 carotenoid 2,2′ diketospirilloxanthin.
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DOI：
10.1016/j.bbalip.2008.12.006
发表时间：
2009-02-01
影响因子：
4.8
作者：
Gerjets, Tanja;Steiger, Sabine;Sandmann, Gerhard
通讯作者：
Sandmann, Gerhard
影响因子：
2.8
作者：
Akahane, J;Rondonuwu, FS;Koyama, Y
通讯作者：
Koyama, Y
影响因子：
3.3
作者：
Desamero, RZB;Chynwat, V;Frank, HA
通讯作者：
Frank, HA
影响因子：
3.6
作者：
Garcia-Asua, G;Cogdell, RJ;Hunter, CN
通讯作者：
Hunter, CN
DOI：
10.1021/jp711946w
发表时间：
2008-08-28
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Cong H;Niedzwiedzki DM;Gibson GN;LaFountain AM;Kelsh RM;Gardiner AT;Cogdell RJ;Frank HA
通讯作者：
Frank HA