Thermoregulatory effects of intracranial norepinephrine injections in goldfish.
Thermoregulatory effects of intracranial norepinephrine injections in goldfish.
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颅内注射去甲肾上腺素对金鱼的体温调节作用。
DOI：
10.1152/ajpregu.1987.253.6.r821
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Panayiotides-Djaferis,H
中科院分区：
文献类型：
--
作者：
Wollmuth,LP;Crawshaw,LI;Panayiotides-Djaferis,H
将导管植入金鱼(Carassius auratus，体重45~90g)的前脑部位，以确定脑内注射去甲肾上腺素(NE)对行为体温调节的影响及其作用部位。在温度梯度下30分钟后，植入的鱼被注射去甲肾上腺素-酒石酸重盐(2.5-500 ng NE)0.2微升0.7%的氯化钠。将5，10，25和50 ng去甲肾上腺素注射到视前核室旁核的前部(NPP，Ref.25)导致选定温度(TSEL)持续的剂量依赖性降低。注射2.5 ng去甲肾上腺素或对照注射100 ng酒石酸对TSEL无影响。其他部位注射的效果，包括脑室内注射，取决于剂量和靠近前NPP；在靠近前NPP的部位，需要更大的剂量，效果变得不一致。在进一步去除的部位，没有观察到对TSEL的影响。包括在NPP和视前核内的更多的尾部部位。我们推测，鱼类的前部NPP是体温调节整合的重要部位，该区域NE释放的增加导致选择更凉爽的水。
Cannulas were implanted into forebrain loci of goldfish (Carassius auratus; 45-90 g) to determine the effects and site of action of intracranial norepinephrine (NE) injections on behavioral thermoregulation. Following 30 min in a thermal gradient, implanted fish were injected with norepinephrine-bitartrate salt (2.5-500 ng NE) in 0.2 microliter 0.7% NaCl. Injections of 5, 10, 25, and 50 ng NE into the anterior aspect of the nucleus preopticus periventricularis (NPP, Ref. 25) led to consistent dose-dependent decreases in selected temperature (Tsel). No effect on Tsel was observed following injections of 2.5 ng NE or control injections of 100 ng tartaric acid. The effects of injections into other loci, including intraventricular injections, were dependent on the dose and proximity to the anterior NPP; at sites adjacent to the anterior NPP, larger doses were required, and the effects became inconsistent. At sites further removed, no effect on Tsel was observed. Included in this category were more caudal sites within the NPP and the nucleus preopticus. We postulate that in fish the anterior NPP is an important locus for thermoregulatory integration and that increased release of NE in this area leads to the selection of cooler water.