LONG-TERM CULTURE OF CAPILLARY ENDOTHELIAL-CELLS
LONG-TERM CULTURE OF CAPILLARY ENDOTHELIAL-CELLS
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DOI：
10.1073/pnas.76.10.5217
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
ZETTER, BR
中科院分区：
文献类型：
--
作者：
FOLKMAN, J;HAUDENSCHILD, CC;ZETTER, BR
来自大鼠、小牛和人的毛细血管内皮细胞已在长期培养中进行。牛毛细血管内皮细胞经连续传代已克隆并维持8个月以上。这种延长的培养是通过使用肿瘤条件培养基，明胶涂层板和原代培养中富集细胞的方法来完成的。培养的牛毛细血管内皮细胞产生因子VIII抗原和血管紧张素转换酶，但不具有韦贝尔-帕拉体。人体细胞确实含有韦贝尔-帕拉德体。毛细血管内皮细胞与主动脉内皮细胞的区别在于它们需要条件培养基。牛毛细血管内皮细胞在正常培养基中生长缓慢，平均倍增时间为67小时，最终死亡。在肿瘤条件培养基中，这些细胞以28小时的倍增时间快速生长，只要肿瘤条件培养基存在，这些细胞就会继续增殖。相反，牛主动脉内皮细胞在正常培养基中生长速度与在肿瘤条件培养基中一样快。这种方法可以产生纯毛细血管内皮细胞，这可能对肿瘤血管生成、转移机制和毛细血管内皮在其他病理状态中的作用的研究有用。
Capillary endothelial cells from rats, calves, and humans, have been carried in long-term culture. Bovine capillary endothelial cells have been cloned and maintained by serial passage for longer than 8 months. This prolonged culture was accomplished by using tumor-conditioned medium, gelatin-coated plates, and a method of enriching cells in primary culture. Cultured bovine capillary endothelial cells produce Factor VIII antigen and angiotensin-converting enzyme, but do not have Weibel-Palade bodies. Human cells do contain Weibel-Palade bodies. Capillary endothelial cells are distinguished from aortic endothelial cells by their requirement for conditioned medium. Bovine capillary endothelial cells in regular medium grow slowly with a mean doubling time of 67 hr and eventually die. In tumor-conditioned medium, these cells grow rapidly with a doubling time of 28 hr and continue to proliferate for as long as the tumor-conditioned medium is present. In contrast, bovine aortic endothelial cells grow as rapidly in regular medium as in tumor-conditioned medium. This method allows the production of pure capillary endothelial cells that may prove useful for studies of tumor angiogenesis, metastatic mechanisms, and the role of capillary endothelium in other pathologic states.