Pepper mild mottle virus coat protein alone can elicit the Capsicum spp. L3 gene-mediated resistance
Pepper mild mottle virus coat protein alone can elicit the Capsicum spp. L3 gene-mediated resistance
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DOI：
10.1094/mpmi.1998.11.12.1253
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发表时间：
1998-12-01
影响因子：
3.5
通讯作者：
Serra, MT
中科院分区：
文献类型：
--
作者：
Gilardi, P;García-Luque, I;Serra, MT
利用基于马铃薯X病毒（PVX）的异源表达系统，在辣椒（LL 3）-L-3植株中表达辣椒轻斑驳病毒（PMMoV-S）外壳蛋白。嵌合病毒定位于接种的叶片中并诱导HR，因此表明影响由L3抗性基因赋予的HR诱导的PMMoV-S外壳蛋白序列存在于外壳蛋白基因中。此外，PMMoV-S外壳蛋白在C，用表达β-葡萄糖醛酸酶（GUS）的质粒基因枪共轰击中国红豆杉叶片该基因导致诱导细胞死亡和宿主防御基因的表达。因此，PMMoV-S的外壳蛋白是辣椒L-3抗性基因介导的HR的激发子。
The pepper mild mottle virus (PMMoV-S) (an L-3 hypersensitive response [HR]-inducer strain) coat protein was expressed in Capsicum chinense ((LL3)-L-3) plants with the heterologous potato virus X (PVX)-based expression system. The chimeric virus was localized in the inoculated leaves and induced the HR, thus indicating that the tobamoviral sequences that affect induction of the HR conferred by the L3 resistance gene reside in the coat protein gene, Furthermore, transient expression of the PMMoV-S coat protein in C, chinense leaves by biolistic co-bombardment with a plasmid expressing the beta-glucuronidase (GUS) gene leads to the induction of cell death and expression of host defense genes. Thus, the coat protein of PMMoV-S is the elicitor of the Capsicum spp, L-3 resistance gene-mediated HR.