A MONOCLONAL-ANTIBODY SPECIFIC FOR DIPLOID EPITHELIAL-CELLS IN DROSOPHILA
A MONOCLONAL-ANTIBODY SPECIFIC FOR DIPLOID EPITHELIAL-CELLS IN DROSOPHILA
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DOI：
10.1038/285403a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
WILCOX, M
中科院分区：
文献类型：
--
作者：
BROWER, DL;SMITH, RJ;WILCOX, M
各种实验结果表明，细胞表面在发育过程中起着重要作用。然而，关于参与发育过程的特定表面分子的信息相对较少。在努力表征可能参与果蝇发育的细胞表面成分的过程中，我们一直在制造抗std的单克隆抗体。melanoyasterimaginal disks2。全变形昆虫的不寻常之处在于，在幼虫组织中散布着一群未分化的想象细胞，这些细胞在变态过程中会形成大多数成虫。我们用作免疫原的想象磁盘是中空的细胞囊；每个圆盘将形成成体角质层的一个特定部分。其他的想象细胞在不同的幼虫器官中被发现为巢或环。我们在这里描述的结果表明，我们分离的一个克隆，DA.1B6，产生针对抗原的抗体，在幼虫中，通常局限于未分化的图像上皮细胞片。这一结果和其他结果表明抗原对果蝇二倍体上皮具有特异性。
Results from various experiments suggest that the cell surface has an important role in development1. However, there is relatively little information on the specific surface molecules involved in developmental processes. In an effort to characterize cell-surface components that may be involved inDrosophiladevelopment, we have been making monoclonal antibodies againstD. melanoyasterimaginal disks2. The holometabolous insects are unusual in that scattered among the larval tissues are groups of undifferentiated imaginal cells which, during metamorphosis, will form most of the adult insect3,4. The imaginal disks, which we use as an immunogen, are hollow sacs of cells; each disk will form a specific part of the adult cuticle. Other imaginal cells are found as nests or rings in various larval organs. We describe here results indicating that one of the clones we have isolated, DA.1B6, makes an antibody against an antigen which, in larvae, is generally restricted to the undifferentiated sheets of imaginal epithelial cells. This and other results indicate that the antigen is specific for the diploid epithelia inDrosophila.