Influence of an L-type SALMFamide neuropeptide on locomotory performance and muscle physiology in the sea cucumber Apostichopus japonicus
Influence of an L-type SALMFamide neuropeptide on locomotory performance and muscle physiology in the sea cucumber Apostichopus japonicus
复制标题
L型SALMF酰胺神经肽对仿刺参运动性能和肌肉生理的影响
DOI：
10.1242/jeb.242566
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发表时间：
2021-10-01
影响因子：
2.8
通讯作者：
Liu, Xiang
中科院分区：
文献类型：
--
作者：
Ding, Kui;Zhang, Libin;Liu, Xiang
SALMFamide家族的神经肽在棘皮动物中作为肌肉松弛剂，并可能影响运动，因为海参的运动行为涉及体壁的交替收缩和伸展，这是在纵肌的控制下进行的。我们评估了l型SALMFamide神经肽（LSA）对Apostichopus japonicus运动性能的影响。我们还利用超高效液相色谱和四极杆飞行时间质谱（UPLC-Q-TOF-MS）研究了纵向肌肉组织的代谢物，以评估LSA作用的潜在生理机制。注射LSA后第4 h，海参的每小时距离、累计时间和移动步数显著增加。平均速度和最大速度分别提高了9.8%和17.8%，平均步幅增加了12.4%。经LSA处理后，纵肌27种代谢物水平发生变化，其中通酸、花生四烯酸、溶磷脂乙醇胺浓度升高，磷脂乙醇胺/磷脂酰胆碱比值改变，是解释LSA对日本刺参运动行为影响的潜在生理机制。
Neuropeptides in the SALMFamide family serve as muscle relaxants in echinoderms and may affect locomotion, as the motor behavior in sea cucumbers involves alternating contraction and extension of the body wall, which is under the control of longitudinal muscle. We evaluated the effect of an L-type SALMFamide neuropeptide (LSA) on locomotory performance of Apostichopus japonicus. We also investigated the metabolites of longitudinal muscle tissue using ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to assess the potential physiological mechanisms underlying the effect of LSA. The hourly distance, cumulative duration and number of steps moved significantly increased in sea cucumbers in the fourth hour after injection with LSA. Also, the treatment enhanced the mean and maximum velocity by 9.8% and 17.8%, respectively, and increased the average stride by 12.4%. Levels of 27 metabolites in longitudinal muscle changed after LSA administration, and the increased concentration of pantothenic acid, arachidonic acid and lysophosphatidy lethanolamine, and the altered phosphatidylethanolamine/ phosphatidylcholine ratio are potential physiological mechanisms that could explain the observed effect of LSA on locomotor behavior in A. japonicus.