Time-resolved luminescent lateral flow assay technology
Time-resolved luminescent lateral flow assay technology
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DOI：
10.1016/j.aca.2008.08.006
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发表时间：
2008-09-26
影响因子：
6.2
通讯作者：
Knotts, Michael
中科院分区：
文献类型：
--
作者：
Song, Xuedong;Knotts, Michael
本文报道了一种将高灵敏度的时间分辨发光技术集成到侧流检测平台中的检测技术，以实现低成本的优异检测性能。我们已经开发出非常明亮，表面功能化和单分散的磷光纳米粒子的长寿命在环境条件下。磷光纳米颗粒已被用于与C反应蛋白（CRP）的单克隆抗体结合，CRP是一种炎症生物标志物。已经开发了使用缀合物的侧流免疫测定装置，用于高灵敏度检测CRP。CRP测定可以在血清中实现<0.2ng mL（-1）的检测灵敏度，线性响应为0.2至200 ng mL（-1）CRP。我们还开发了一种低成本的时间分辨发光阅读器的侧流免疫测定（LFIA）设备。读取器不使用昂贵的带通滤波器，并且在固体基底（例如硝酸纤维素膜）上仍然提供非常低的检测背景和高检测灵敏度。该阅读器可以检测硝酸纤维素膜条上捕获的小于2.5 ng的磷光颗粒，线性检测动态范围超过三个数量级。这项技术应该有许多应用，从临床诊断、检测化学和生物战剂到食品和环境监测。(c)2008 Elsevier B. V.保留所有权利。
We here report a detection technology that integrates highly sensitive time-resolved luminescence technique into lateral flow assay platform to achieve excellent detection performance with low cost. We have developed very bright, surface-functionalized and mono-dispersed phosphorescent nanoparticles of long lifetime under ambient conditions. The phosphorescent nanoparticles have been used to conjugate with monoclonal antibody for C-reactive protein (CRP), an inflammatory biomarker. Lateral flow immunoassay devices have been developed using the conjugate for highly sensitive detection of CRP The CRP assay can achieve a detection sensitivity of < 0.2 ng mL(-1) in serum with a linear response from 0.2 to 200 ng mL(-1) CRP. We have also developed a low cost time-resolved luminescence reader for the lateral flow immunoassay (LFIA) devices. The reader does not use expensive band pass filter and still provide very low detection background and high detection sensitivity on solid substrates such as nitrocellulose membranes. The reader can detect less than 2.5 ng phosphorescent particles captured on a nitrocellulose membrane strip with more than three orders of magnitude linear detection dynamic range. The technology should find a number of applications, ranging from clinical diagnostics, detection of chemical and biological warfare agents, to food and environmental monitoring. (c) 2008 Elsevier B.V. All rights reserved.