Gangliosides as markers for murine lymphocyte subpopulations
Gangliosides as markers for murine lymphocyte subpopulations
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神经节苷脂作为小鼠淋巴细胞亚群的标记物
DOI：
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发表时间：
1976
影响因子：
15.3
通讯作者：
D. Marcus
中科院分区：
文献类型：
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作者：
K. Stein;G. Schwarting;M. Naiki;D. Marcus
GM1神经节苷脂抗体用于研究小鼠淋巴细胞群。在A、AKR和BALB/c小鼠中，抗GM1与胸腺细胞和外周T细胞反应。免疫荧光法研究的这种抗GM1的反应性与Thy-1类型无关，而且似乎与针对asialo GM1和GD1b的交叉反应抗体的反应性有关，而不是GM1本身。此外，在C3H小鼠、裸鼠和裸鼠杂合子的外周血淋巴细胞中，约有26%的淋巴细胞可与抗GM1和抗免疫球蛋白反应。这一亚群在A、AKR和BALB/c小鼠中少量发现。这些研究表明，针对一种化学定义的抗原的抗体可以用来识别许多品系的小鼠的T细胞，并可能描绘出以前未识别的淋巴细胞亚群。
Antibodies to GM1 ganglioside were used to study murine lymphocyte populations. In A, AKR, and BALB/c mice, anti-GM1 reacts with thymocytes and peripheral T cells. This reactivity of anti-GM1, studied by immunofluorescence, is independent of Thy-1 type and appears to be related to the reactivity of cross-reacting antibodies to asialo GM1 and GD1b, rather than GM1 itself. In addition, a subpopulation of lymphocytes reacting with anti-GM1 and anti-immunoglobulin has been found in approximately 26% of the peripheral lymphocytes of C3H mice, nude mice, and nude heterozygotes. This subpopulation is found in small numbers in A, AKR, and BALB/c mice. These studies demonstrate that antibodies to a chemically defined antigen can be used to identify T cells in many strains of mice and may delineate previously unrecognized lymphocyte subpopulations.