Prevalence of the CCR5Δ32 mutation in Brazilian populations and cell susceptibility to HIV-1 infection
Prevalence of the CCR5Δ32 mutation in Brazilian populations and cell susceptibility to HIV-1 infection
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DOI：
10.1007/s00439-002-0747-x
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发表时间：
2002-07-01
期刊：
影响因子：
5.3
通讯作者：
Galvao-Castro, B
中科院分区：
文献类型：
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作者：
Grimaldi, R;Shindo, N;Galvao-Castro, B
我们调查了巴西不同地区种族人群中CCR 5Delta 32突变的发生情况，并测试了突变外周血单核细胞（PBMC）对HIV-1体外感染的抵抗力。未感染的非洲后裔中杂合子患病率为5.3%，HIV-1阳性个体中杂合子患病率为8.8%（两个人群均没有Delta 32/Delta 32）。德国后裔为11%杂合子和1% Delta 32/Delta 32。美洲印第安人完全是CCR 5/CCR 5。未感染杂合子的PBMC对R5-HIV-1株有部分耐药，但对X4病毒无耐药。HIV-1阳性的CCR 5/CCR 5比杂合子细胞具有更高的病毒载量。
We investigated the occurrence of the CCR5Delta32 mutation in various regional ethnic groups in Brazil and tested the resistance of mutant peripheral blood mononuclear cells (PBMCs) to infection by HIV-1 in vitro. The heterozygous prevalence was 5.3% in uninfected African descendents and 8.8% in HIV-1-positive individuals (neither population had Delta32/Delta32). German descendents were 11% heterozygous and 1% Delta32/Delta32. Amerindians were exclusively CCR5/CCR5. Heterozygous uninfected PBMCs showed partial resistance to R5-HIV-1 strains in vitro, but no resistance to X4 virus. HIV-1-positive CCR5/CCR5 had higher viral loads than did heterozygous cells.