Hyperspectral optical diffraction tomography
Hyperspectral optical diffraction tomography
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DOI：
10.1364/oe.24.002006
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发表时间：
2016-02-08
期刊：
影响因子：
3.8
通讯作者：
Park, YongKeun
中科院分区：
文献类型：
--
作者：
Jung, JaeHwang;Kim, Kyoohyun;Park, YongKeun
在这里，我们提出了一种新的显微技术，用于测量波长相关的三维（3-D）分布的折射率（RI）的微观样品在可见光波长。采用3-D定量相位显微镜技术与波长扫描源，3-D RI断层图，在450 - 700 nm的范围内获得了几个纳米的光谱分辨率。通过测量聚苯乙烯珠、人红细胞和肝细胞的高光谱3-D RI断层图像，证明了该技术的能力。结果表明，无标记的分子特异性的生物样品的3-D断层扫描的潜力。(C)2016美国光学学会
Here, we present a novel microscopic technique for measuring wavelength-dependent three-dimensional (3-D) distributions of the refractive indices (RIs) of microscopic samples in the visible wavelengths. Employing 3-D quantitative phase microscopy techniques with a wavelength-swept source, 3-D RI tomograms were obtained in the range of 450 - 700 nm with a spectral resolution of a few nanometers. The capability of the technique was demonstrated by measuring the hyperspectral 3-D RI tomograms of polystyrene beads, human red blood cells, and hepatocytes. The results demonstrate the potential for label-free molecular specific 3-D tomography of biological samples. (C) 2016 Optical Society of America