Polycystin-2 associates with the polycystin-1 homolog, suREJ3, and localizes to the acrosomal region of sea urchin spermatozoa
Polycystin-2 associates with the polycystin-1 homolog, suREJ3, and localizes to the acrosomal region of sea urchin spermatozoa
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DOI：
10.1002/mrd.20033
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发表时间：
2004-04-01
影响因子：
2.5
通讯作者：
Vacquier, VD
中科院分区：
文献类型：
--
作者：
Neill, AT;Moy, GW;Vacquier, VD
Polycystin-2 是 2 型常染色体显性多囊肾病中突变的蛋白质，是一种具有非选择性阳离子通道活性的完整跨膜蛋白。在这里，我们报告了海胆精子多囊蛋白-2 (suPC2) 的同源物。与其他多囊蛋白-2 家族成员一样，suPC2 是一种六次跨膜蛋白，含有 C 端胞质 EF 手和卷曲螺旋结构域。该蛋白质仅定位于精子顶体囊泡上方的质膜。该定位与先前报道的海胆 PC1 同源物 suREJ3 的定位一致。免疫共沉淀显示 suPC2 和 suREJ3 在膜中结合。 suPC2 的位置表明它可能充当介导精子顶体反应的阳离子通道。 PC2 通道的低阳离子选择性可以解释表明 Na+ 和 Ca2+ 可能在顶体反应期间通过同一通道进入海胆精子的数据。 (C) 2004 Wiley-Liss, Inc.
Polycystin-2, the protein mutated in type 2 autosomal dominant polycystic kidney disease, is an integral transmembrane protein with nonselective cation channel activity. Here we report on the sea urchin sperm homolog of polycystin-2 (suPC2). Like other polycystin-2 family members, suPC2 is a six-pass transmembrane protein containing C-terminal cytoplasmic EF hand and coiled-coil domains. The protein localizes exclusively to the plasma membrane over the sperm acrosomal vesicle. This localization coincides with the previously reported localization of the sea urchin PC1 homolog, suREJ3. Co-immunoprecipitation shows that suPC2 and suREJ3 are associated in the membrane. The location of suPC2 suggests that it may function as a cation channel mediating the sperm acrosome reaction. The low cation selectivity of PC2 channels would explain data indicating that Na+ and Ca2+ may enter sea urchin sperm through the same channel during the acrosome reaction. (C) 2004 Wiley-Liss, Inc.