Bacterial antagonist as seed treatment to control leaf blight disease of bambara groundnut (Vigna subterranea)
Bacterial antagonist as seed treatment to control leaf blight disease of bambara groundnut (Vigna subterranea)
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DOI：
10.1007/s11274-005-2820-9
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发表时间：
2006-01-01
影响因子：
4.1
通讯作者：
Kanjanamaneesathian, M
中科院分区：
文献类型：
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作者：
Pengnoo, A;Wiwattanapattapee, R;Kanjanamaneesathian, M
土壤样品取自泰国南部48块种植了bambara花生（Vigna subtaguea）或花生（Arachis hypogeae）的田地。芽孢杆菌使用土壤稀释平板和热处理分离以筛选产生内孢子的细菌。在342株芽孢杆菌中，用双培养法测定了168个菌株对慢生根瘤菌NC-92菌株的拮抗性。进一步检测发现16株芽孢杆菌。对黄麻花生叶枯病病原菌立枯丝核菌菌丝生长有抑制作用。在这些分离物中，芽孢杆菌属（Bacillus spp.）TRV 9-5-2对R.索拉尼该分离株后来被鉴定为B。坚定。B的粉末制剂。通过混合细菌内生孢子、淀粉、羧甲基纤维素钠（SCMC）和聚乙烯吡咯烷酮（PVP）来开发firmus。制剂含有范围为10（8）至10（10）c.f.u./ g，并且在室温（26-32 ℃）下储存1年后，所有制剂中的细菌存活力仍然很高。体外抑菌试验表明，所有制剂均具有较好的抑菌效果。采用双元培养技术，种子处理试验表明，该制剂不会引起花生苗形异常，对花生种子萌发无影响。
Soil samples were taken from 48 fields in the southern part of Thailand in which either bambara groundnut (Vigna subterranea) or groundnut (Arachis hypogeae) had been planted. Bacillus spp. were isolated using soil dilution plates and heat treatment to screen for endospore-producing bacteria. Among 342 Bacillus spp. isolates tested, 168 isolates were not antagonistic to Bradyrhizobium sp. strain NC-92 using dual culture technique. Further testing found 16 isolates of Bacillus spp. had the ability to inhibit mycelial growth of Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut. Among these isolates, Bacillus spp. isolate TRV 9-5-2 had the greatest activity in anti-microbial tests against R. solani. This isolate was later identified as B. firmus. A powder formulation of B. firmus was developed by mixing bacterial endospores, talcum, sodium carboxymethylcellulose (SCMC) and polyvinylpyrolidone (PVP). The formulations contained bacterial levels ranging from 10(8) to 10(10) c.f.u./g and the viability of bacteria in all formulations remained high after 1 year storage at room temperature (26-32 degrees C). All formulations showed satisfactory effectiveness in vitro in suppressing mycelial growth of R. solani using dual culture technique. The application of formulations as seed treatment showed that these formulations did not cause abnormality of seedling shape and had no effect on the germination of bambara groundnut seeds.