Isolation and characterisation of surface active compound-producing bacteria from hydrocarbon-contaminated environments
Isolation and characterisation of surface active compound-producing bacteria from hydrocarbon-contaminated environments
复制标题
DOI：
10.1016/j.ibiod.2009.05.003
复制
发表时间：
2009-10
影响因子：
4.8
通讯作者：
C. Ruggeri;A. Franzetti;G. Bestetti;P. Caredda;Paolo La Colla;M. Pintus;S. Sergi;E. Tamburini
中科院分区：
文献类型：
--
作者：
C. Ruggeri;A. Franzetti;G. Bestetti;P. Caredda;Paolo La Colla;M. Pintus;S. Sergi;E. Tamburini
从烃类污染环境中分离出能产生表面活性物质的细菌。通过16 S rRNA基因分析，对分离菌株的系统发育多样性进行了评价。在代表18种不同细菌属的菌株上测定生物乳化剂和生物表面活性剂的生产。贪铜菌BSNC 28 C产生胞外生物表面活性剂，可将培养基的表面张力降低至37.1 mN m−1。16株，属于11个不同的属，释放细胞外乳化剂能够稳定油-水乳液。其中，菌株慢生根瘤菌BSNC 30 A和Bosea sp.BSNC5B显示出与合成表面活性剂相当的乳化活性。总的来说，在这项工作中表征的新型SAC生产菌株显示出未来经济高效的工业规模生物技术工艺发展的有前途的特征。
Bacteria able to produce surface active compounds (SACs) were isolated from hydrocarbon-contaminated environments. The phylogenetic diversity of the isolates was evaluated by 16S rRNA gene analysis. The production of bioemulsifiers and biosurfactants was determined on strains representative of 18 different bacterial genera. Cupriavidus sp. BSNC28C produced extracellular biosurfactants which reduce the surface tension into the culture medium up to 37.1 mN m−1. Sixteen strains, belonging to 11 different genera, released extracellular emulsifiers able to stabilise oil–water emulsions. Among them, the strains Bradyrhizobium sp. BSNC30A and Bosea sp. BSNC5B showed emulsification activities comparable to those of synthetic surfactants. Overall, the novel SAC-producing strains characterised in this work display promising features for the future development of economically efficient industrial-scale biotechnological processes.