EVALUATION IN TOBACCO OF THE ORGAN SPECIFICITY AND STRENGTH OF THE ROLD PROMOTER, DOMAIN-A OF THE 35S PROMOTER AND THE 35S(2) PROMOTER
EVALUATION IN TOBACCO OF THE ORGAN SPECIFICITY AND STRENGTH OF THE ROLD PROMOTER, DOMAIN-A OF THE 35S PROMOTER AND THE 35S(2) PROMOTER
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DOI：
10.1007/bf01973757
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发表时间：
1995-11-01
影响因子：
3
通讯作者：
TEPFER, M
中科院分区：
文献类型：
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作者：
ELMAYAN, T;TEPFER, M
为了研究发根农杆菌的rolD启动子在植物中的表达，我们构建了嵌合基因，将gusA（uidA）标记基因的编码区置于两个不同长度的rolD启动子片段的控制下。用两种基因获得了类似的结果。在转化的R（1）后代植物中进行表达研究。在成熟的转化烟草植株中，rolD-gus基因在根中强烈表达，而在茎和叶中表达水平低得多。这种表达模式被传递到后代，虽然在根中的表达水平相对于在叶中的表达水平的比率在幼苗中低得多。rolD-gus转化体中的根特异性程度小于用CaMV 35 S启动子的结构域A构建的基因domA-gus，但根表达水平远高于后者基因。然而，rolD-gus基因的表达水平低于具有双结构域B的35 S启动子的gus基因，35 S（2）-gus。rolD-gus基因在根中的表达具有独特的模式，与其他两个基因相比，在根伸长区和维管组织中观察到最强的GUS活性，而在根尖中的GUS活性要低得多。
In order to study the expression in plants of the rolD promoter of Agrobacterium rhizogenes, we have constructed chimaeric genes placing the coding region of the gusA (uidA) marker gene under control of two rolD promoter fragments of different length. Similar results were obtained with both genes. Expression studies were carried out in transformed R(1) progeny plants. In mature transformed tobacco plants, the rolD-gus genes were expressed strongly in roots, and to much lower levels in stems and leaves. This pattern of expression was transmitted to progeny, though the ratio of the level of expression in roots relative to that in leaves was much lower in young seedlings. The degree of root specificity in rolD-gus transformants was less than that of a gene constructed with domain A of the CaMV 35S promoter, domA-gus, but the level of root expression was much higher than with the latter gene. However, the level of expression of the rolD-gus genes was less than that of a gus gene with a 35S promoter with doubled domain B, 35S(2)-gus. The rolD-gus genes had a distinctive pattern of expression in roots, compared to that of the two other genes, with the strongest GUS activity observed in the root elongation zone and in vascular tissue, and much less in the root apex.